. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Lopholithodes foraminatus * 1 / O ⢠*2 â. 5 3 I 0 5 3 I 70 75 pH pH Figure 1. The effect of temperature on hemocyanin oxygen affinity (log P50) and cooperativity (n50) as a function of pH in four "cold" water crabs. Cancer anthonyi. Cancer magister, Cancer gracilis, and Lopholithodes foraminatus. P50 and n50 were determined at 5°C (a), 15°C (A), 25°C (â¢), and 35"C (O). using extinction coefficients reported by Nickerson and Van Holde (1971)] was combined wit
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Lopholithodes foraminatus * 1 / O ⢠*2 â. 5 3 I 0 5 3 I 70 75 pH pH Figure 1. The effect of temperature on hemocyanin oxygen affinity (log P50) and cooperativity (n50) as a function of pH in four "cold" water crabs. Cancer anthonyi. Cancer magister, Cancer gracilis, and Lopholithodes foraminatus. P50 and n50 were determined at 5°C (a), 15°C (A), 25°C (â¢), and 35"C (O). using extinction coefficients reported by Nickerson and Van Holde (1971)] was combined with serum and stirred at room temperature for 1 h. The lipid-detergent com- plex was removed by adding 1 g Bio-Beads (SM-2 20-50 mesh. BioRad Co.)/g He and stirring for another h. The Bio-Beads were then removed by filtration through cotton. Calcium was removed from samples of C. anthonyi hemolymph by dialysis overnight against two changes of 500 mmol/1 NaCl and 1 mmol/1 EGTA (ethyleneglycol- bis-N,N-tetra-acetic acid) in a ratio of 1 volume of sam- ple:500 volumes of dialysis medium. Results In five of the eight species studied (the exceptions be- ing S. conspicua, L. foraminatus, and L. polyphemus) temperature sensitivity of Hc02 affinity is generally low- est at the highest temperatures (Figs. 1, 2, 3). In the van't Hoff plots (Fig. 3) this is represented by slopes which ap- proach zero. In all species the Bohr coefficients (A log P50/ApH) differ significantly from zero (P < .01) throughout the temperature range. In the five cold water species, temperature sensitivity of P5o is absent or, in //. corrugata, lowest between 25 and 35°C (Figs. 1, 2; Table I). This is most easily seen in the van't Hoff plots (Fig. 3). Hc02 affinity in C gracilis and C. magister also does not change significa 5 to 15°C at the physiological pH () (Table surpris- ingly, a large positive value for AH was foi L. fora- minatus between 5 and 15°C. However is calcu- lated for the
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
