. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 'ercent of Uninfected Untreated Control Cultur D Dose (pg/ml) Percent of Protection 1CT1 x 10~1 10° ° +1 +1 +2 10+2 200 150 100 50 0 -50 400 I I I I 100 200 300 400 500 600 700. 0 100 200 300 400 500 600 700 Time (sec) Figure 1. Summary of the antiviral propertv of MAF in CEM lymphoblastoid cells infected in//? human immunodeficiency virus (H1V-1} (a, b). Sensorgrarns indicate MAF inhibition of rgp!20-rCD4 binding bv MAF ft',
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 'ercent of Uninfected Untreated Control Cultur D Dose (pg/ml) Percent of Protection 1CT1 x 10~1 10° ° +1 +1 +2 10+2 200 150 100 50 0 -50 400 I I I I 100 200 300 400 500 600 700. 0 100 200 300 400 500 600 700 Time (sec) Figure 1. Summary of the antiviral propertv of MAF in CEM lymphoblastoid cells infected in//? human immunodeficiency virus (H1V-1} (a, b). Sensorgrarns indicate MAF inhibition of rgp!20-rCD4 binding bv MAF ft', d), whereas synthetic sitlfated andpyruvylated constructs show no inhibition (e, f). (a, b) Results of forma-an XTT assays for viral inhibitory properties of MAF derivatives. Round black dots and diamonds depict infected treated culture and Uninfected treated culture respectively. MAF gave >50% protection at a dose as low as (EC50), while demonstrating little or no toxic effects. Lower dotted line demonstrates viral pathogenic effect in infected untreated culture. Straight dashes are 50% ami 100% reference lines, (c-f) Sensorgrarns which schematize the relative binding affinities of self and non-self MAF binding epitopes. The small letters that accompany each set ofcun'es represent the following: a = rpg!20 — rCD4 binding, b = rpg/20 + putative inhibitor — rCD4 interaction, c = putative inhibitor - rCD4 reaction. The constants for these reactions are given b\ the terms k:l and kj. where k:l refers to the association constant and ktl describes the dissociation constant. The values for rpg 120 at the stated concentration were X Iff1 and x 10 4 respectively and indicated a strong binding affinity between rpg/20 and rCD4 (a curves). The reactions did not differ appreciably when gp!20 was in mixture with the sit/fate or pyruvylated compounds (b cun'es). However, both MAF I and MAF ds were reactive as inhibitory compounds. MAFI a/so possessed a strong affi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
