. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ^ THE VITELLIXE COAT OF THE MYTILUS EGG. I. NORMAL STRUCTURE AND EFFECT OF ACROSOMAL LYSIN JEAN C. DAN Ochanoinizn University. Tokyo, and The Misuki Marine Bi/ilouical Station. Miura-Slu. Japan The first study of a lysin extractable from Mytilus spermatozoa was made by Berg (1950), who distinguished two types of lytic activity : a "membrane-dissolving" action, which was described as attacking an outer layer of the egg surface made microscopically visible by plasmolyzing the eggs, and a "cement-dissolving"
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ^ THE VITELLIXE COAT OF THE MYTILUS EGG. I. NORMAL STRUCTURE AND EFFECT OF ACROSOMAL LYSIN JEAN C. DAN Ochanoinizn University. Tokyo, and The Misuki Marine Bi/ilouical Station. Miura-Slu. Japan The first study of a lysin extractable from Mytilus spermatozoa was made by Berg (1950), who distinguished two types of lytic activity : a "membrane-dissolving" action, which was described as attacking an outer layer of the egg surface made microscopically visible by plasmolyzing the eggs, and a "cement-dissolving" activity, which causes the first two blastomeres to become partially or completely separated. Wada, Collier and Dan (1956) showed that the lysin in question is a component of the intact acrosome which is released into the medium when the acrosome is induced to react. Col win and Col win (1960a, b) have also investigated the effect of a lysin extracted from the spermatozoa of Hydroides hexagonus on the egg of this species. Using thin sections and electron microscopy, they found that the lysin dissolves the middle, and major, component of the thick vitelline coat investing the cytoplasmic surface, although it appears not to affect the outer and inner borders of this envelope. A report concerning the structure of the Mytilus cdulis ''egg membrane" has recently been published by Mancuso (1960). This author used a fixing solution which included formalin, acetone, acetic acid and sometimes chromic acid, as well as osmium tetroxide; the images observed in the electron microscope after this fixation led him to certain conclusions which differ considerably from those reached in this study. None of these differences, however, is so radical that it cannot be attributed to the effect of the fixative. The present investigation was undertaken to observe the fine structure of the Mytilus egg surface and determine in detail how the acrosomal lysin affects it after fertilization, particul
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
