. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. totheca thamensis, and Thalassiosira decipiens. Of all these species the cultivation of Nitz- schia closterium /. minu- tissima is more widely practised in many lab- oratories both in the United States and in Europe than that of any other diatom. This is primarily due to its small size and its ability to remain in suspension for a long period of time, these qualities rendering it very useful as food for small plank- tonic organisms. Light and temperature are t
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. totheca thamensis, and Thalassiosira decipiens. Of all these species the cultivation of Nitz- schia closterium /. minu- tissima is more widely practised in many lab- oratories both in the United States and in Europe than that of any other diatom. This is primarily due to its small size and its ability to remain in suspension for a long period of time, these qualities rendering it very useful as food for small plank- tonic organisms. Light and temperature are the two principal physical factors which govern the rate of prop- agation of diatoms. The growth of cultures left in the laboratory and subject to rather wide fluc- tuations in temperature and intensity of illumination are greatly affected by these changes. Temperatures above 22° C. are obviously harmful to a Nitzschia culture and when the summer heat approaches 300, as hap- pens regularly in the author's laboratory of the U. S. Bureau of Fisheries, they may perish. To avoid this difficulty and to keep the cultures in health, both temperature and illumination should be kept constant. The following easily constructed arrangement has been successfully used by the author. Culture flasks are kept in a cabinet (Fig. 34), 60" Fig. 34.—The cabinet for diatom cultures. A, air switch; C, dry cells; E, relay; F, fan; H, small heater; M, metastatic temperature controller; P. pipes to refrigeration plant; R, refrigeration unit; S, wall switch; T, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Galtsoff, Paul Simon, 1887-. update forthcoming
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