. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SHARK LYMPHOMYELOID TISSUE 185. FIGURE 2. Light microscopy of the Leydig organ of Etmopterus. (A): Epon-embedded section, about 1 ^/m thick and stained with toluidine blue. In the middle an eosinophilic granulocyte (E). Lymphocytes (L) and heterophilic granulocytes (H) are seen around the eosinophilic cell. At the bottom a typical leptomeric cell (Le) with invaginated nucleus. Oil immersion. Bar == 10 ^m. (B): Azan-stained paraffin section. In the middle of the figure a group of non-granulated cells, mainly lymphocytes. Abov
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SHARK LYMPHOMYELOID TISSUE 185. FIGURE 2. Light microscopy of the Leydig organ of Etmopterus. (A): Epon-embedded section, about 1 ^/m thick and stained with toluidine blue. In the middle an eosinophilic granulocyte (E). Lymphocytes (L) and heterophilic granulocytes (H) are seen around the eosinophilic cell. At the bottom a typical leptomeric cell (Le) with invaginated nucleus. Oil immersion. Bar == 10 ^m. (B): Azan-stained paraffin section. In the middle of the figure a group of non-granulated cells, mainly lymphocytes. Above and below these cells there are eosinophilic granulocytes (dark cells). Bar = 10 Mm. small lymphocytes. In Giemsa-stained histological sections the tissue appears as a mosaic of red-stained granulocytes and bluish non-granulated cells. Electron microscopy Most cells of the Leydig organ show ultrastructural characteristics resembling those of blood cells from various vertebrates and even invertebrates (Bessis, 1973; Mattisson and Fa'nge, 1977). The same types of leucocytes as identified by light microscopy are also found by the electron microscope, but the more structural details obtained are important for a further identification of the leucocytes. Eosinophilic granulocytes. On electron micrographs the granules measure up to ^urn in diameter and have a spheric form (Fig. 4). In contrast to generally described eosinophils of higher vertebrates those of the Etmopterus Leydig organ contain granules without a crystalline core. The periphery of the granules often has an electron lucent frame. Compared with the heterophilic cells described below the eosinophils are less densely packed with granules. Surrounding the granules a rough endoplasmic reticulum is often found (Fig. 4). The eosinophils seem to make up only about 5% of the total number of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readabil
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
