. Biological structure and function; proceedings. Biochemistry; Cytology. FUNCTION OF FLAVOENZYMES IN ELECTRON TRANSPORT I43 TABLE III ESTERIFICATION OF PHOSPHATE DURING THE OxiDATION OF Substrate in the Presence of Amytal and \"itamix Kg (Conover and Ernster unpublished) Experimental conditions as in Table II. Substrates were added in a final concentration of o-oi M. P 0 ratio Substrate None \' itamin K., Amytal Vitamin K3 + amytal glutamate iS-OH-butyrate succinate 2-63 2-14 1-78 2-46 1-40 1-65 1-53 1-45 1-04 I-I9 The antimycin A-sensitivity of the Yitamin Ky-induced respiration in the
. Biological structure and function; proceedings. Biochemistry; Cytology. FUNCTION OF FLAVOENZYMES IN ELECTRON TRANSPORT I43 TABLE III ESTERIFICATION OF PHOSPHATE DURING THE OxiDATION OF Substrate in the Presence of Amytal and \"itamix Kg (Conover and Ernster unpublished) Experimental conditions as in Table II. Substrates were added in a final concentration of o-oi M. P 0 ratio Substrate None \' itamin K., Amytal Vitamin K3 + amytal glutamate iS-OH-butyrate succinate 2-63 2-14 1-78 2-46 1-40 1-65 1-53 1-45 1-04 I-I9 The antimycin A-sensitivity of the Yitamin Ky-induced respiration in the amytal-blocked system indicated that the electrons mediated by vitamin K3 may enter the respiratory chain at the level of cytochrome b. This point could be tested with the double-beam spectrophotometer, 400- 300- 200 434-490 m// cyt. b reduction. Jf=00lcm' Fig. I. Effect of amytal and vitamin Kj on the oxygen consumption and on the reduction of cytochrome c during the oxidation of glutamate (Conover and Ernster, unpublished). The medium contained 001 m triethanolamine buffer, pH 7-4, o-oi M Pj, pH 74, 0004 .M MgCL, 0065 M KCl, and o-i M sucrose. Mitochondria from 200 mg. rat liver were used. The amounts of additions were as follows: 2-0 /amoles L-glutamate, o-8 /^mole ADP, 2-0 /imoles amytal, and 0-04 ^mole vitamin K3. Final volume, i -2 ml. Temperature, 25 ^. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
Size: 1250px × 2000px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
