. A manual of bacteriology. Bacteriology. THE CULTIVATION OF BACTERIA. 99 Esmarch's Roll-tubes.—Either gelatin or agar may be used for roll-tubes, but if the agar is freshly made, it does not adhere well to the walls of the tuber. The dilutions in tubes i, 2, and 3 are made as above. Tubes containing a rather small amount of the culture-medium are more convenient. The tubes may be capped with a rubber cap and revolved in a dish of ice water, or under the water tap or, a still better method employed first by W. D. Booker, a block of ice should be at hand, and, with a tube filled with hot water
. A manual of bacteriology. Bacteriology. THE CULTIVATION OF BACTERIA. 99 Esmarch's Roll-tubes.—Either gelatin or agar may be used for roll-tubes, but if the agar is freshly made, it does not adhere well to the walls of the tuber. The dilutions in tubes i, 2, and 3 are made as above. Tubes containing a rather small amount of the culture-medium are more convenient. The tubes may be capped with a rubber cap and revolved in a dish of ice water, or under the water tap or, a still better method employed first by W. D. Booker, a block of ice should be at hand, and, with a tube filled with hot water and lying horizon-. FlG. 35.—Manner of making Esmarch roll-tube. tally, a hollow of the size of the test-tube should be melted on the upper surface of the ice. In this hollow place the tube of liquified gelatin or agar; roll it rapidly with the hand, taking care that the culture-medium does not run toward the neck as far as the cotton plug. The medium is spread in a uniform manner around the inside of the tube, where it becomes solidi- fied. Gelatin roll-tubes must be kept in a place so cool that there is no danger of their melting; in handling them they are to be held by the portion of the tube into which the cot- ton plug projects, so that the warmth of the hand may not. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Williams, Herbert Upham, 1866-; Bolton, Benjamin Meade, 1857-1929. Philadelphia, P. Blakiston's Son & Co.
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1908
