. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 116 P. G. GREENWOOD ET AL. 3 50- Control probes Mucus-coated probes (6). FSW FSW + NANA Figure 1. Number of nematocysts discharged into control probes or into probes coated with mucus from individuals of Aeolulia papillosa that had been feeding on Metridit/ni xcnile. Mucus-coated probes were used to probe tentacles of the sea anemone M. senile in both filtered seawater (FSW) and FSW containing 1CT7 M N-acetylneuraminic acid (FSW + NANA), and compared against control probes of gelatin only. The numbers in parentheses are the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 116 P. G. GREENWOOD ET AL. 3 50- Control probes Mucus-coated probes (6). FSW FSW + NANA Figure 1. Number of nematocysts discharged into control probes or into probes coated with mucus from individuals of Aeolulia papillosa that had been feeding on Metridit/ni xcnile. Mucus-coated probes were used to probe tentacles of the sea anemone M. senile in both filtered seawater (FSW) and FSW containing 1CT7 M N-acetylneuraminic acid (FSW + NANA), and compared against control probes of gelatin only. The numbers in parentheses are the numbers of anemones used (control probes) or the numbers of nudibranchs used (mucus-coated probes) per test. cysts into mucus-coated probes from unswabbed nudi- branchs was compared to nematocyst discharge into mucus- coated probes from swabbed nudibranchs and into control probes. Analysis was done by ANOVA and with Scheffe's test for pairwise comparisons. We also investigated whether the mucus of M. senile individuals inhibited discharge from other individuals of M. senile held in separate containers. Individual sea anemones were transferred to filtered seawater. allowed to recover for 10 min, transferred to fresh filtered seawater again, allowed to recover for 10 mm. and finally transferred to a dry, clean glass dish. The sea anemone secreted mucus for 10 min and was removed from the dish. The mucus was removed from the dish and stored in sterile microcentrifuge tubes. Gelatin probes were placed into the mucus for 2 min and used to probe different individuals of M. .senile. Three probes were coated with mucus from each of tour anemones. The mean number of discharged nematocysts in the mucus-coated probes was compared to that number in control probes with no mucus. Data were analy/ed using Student's / tests as described above. Results Does a luulihraneli's nu/ens inliihit the neinaloeyst t!i-,< Inline ti'din the lentaeles of its prey species'.' When tentacles of the sea ane
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