. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 366 Hydrophobia, Lyssa, or Rabies from the unstructured Negri bodies observed with great frequency in the rabid guinea-pig brain. In a few instances these forms con- tained a blue-staining central ring or point, and closely resembled the structured forms of Negri bodies. The normal guinea-pig brain inoculated with rabid material, street or fixed virus, incubated in the same manner, showed the same structures. The brains of guinea- pigs dying of street vi
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 366 Hydrophobia, Lyssa, or Rabies from the unstructured Negri bodies observed with great frequency in the rabid guinea-pig brain. In a few instances these forms con- tained a blue-staining central ring or point, and closely resembled the structured forms of Negri bodies. The normal guinea-pig brain inoculated with rabid material, street or fixed virus, incubated in the same manner, showed the same structures. The brains of guinea- pigs dying of street virus and rabbits dying of fixed virus, incubated in small fragments, gave no development of Negri bodies in blood plasma, beyond the small structured and unstructured forms, al- though in one preparation the ganglion cells appeared to be living at the end of twenty-one days' incubation. Cultivation.—Attempts to cultivate Negri bodies were made by Moon,* but the success of his attempts seemed doubtful. The first. Fig. 136.—From rabbit "fixed-virus" brain; a, b, c, d,f, and i, tj^pes of Negri bodies seen at deatli of rabbit; c, g, h, and/, apparent multiplication and segmen- tation of the bodies after three days at 24°C. Drawing made from smears stained by Giemsa's method and magnified about 2000 diameters (Williams, in Jour. Am. Med. Assoc). claim to successful cultivation of the Negri bodies was made by The cultivation was done according to his already suc- cessful method for Spirochaeta of various kinds. Large, small and dividing bodies appeared in the culture fluid, after inoculation with a fragment of nervous tissue from various animals with infection fol- lowing inoculation with street virus and "fixed" virus. But Wil- liams! at once pointed out that there is no certainty that the bodies increased in numbers in the cultures, though Noguchi says that they reappear in new cultures "through many ; Noguchi's * "Jour,
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
