. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CA LOCALIZATION IN CASSIOPEA XAMACHANA 283 uals over an 8-week period. Paired Student's t tests were used to compare algal density and CA activity in field- condition, week-3 bleached, and week-5 repopulated sam- ples. A one-way ANOVA was run for algal number and CA activity in the following comparisons: of bell margin versus arm tissue; between and among oral, aboral, and mesogleal tissue: and of tissue samples across a transect from bell margin to manubrium. Scheffe PLSD post hoc tests were run on all ANOVAs (Sokal and Roh
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CA LOCALIZATION IN CASSIOPEA XAMACHANA 283 uals over an 8-week period. Paired Student's t tests were used to compare algal density and CA activity in field- condition, week-3 bleached, and week-5 repopulated sam- ples. A one-way ANOVA was run for algal number and CA activity in the following comparisons: of bell margin versus arm tissue; between and among oral, aboral, and mesogleal tissue: and of tissue samples across a transect from bell margin to manubrium. Scheffe PLSD post hoc tests were run on all ANOVAs (Sokal and Rohlf. 1995). All statistical analyses were performed using Stat-View (SAS Institute, Cary, NO. Results Localization of carbonic anliydnise via DNSA-CA fluorescence Frozen sections of Cassiopea xamacluum bell margin incubated with 50 /xA/ DNSA showed intense labeling of oral and aboral epithelial layers. By comparison, there was no significant DNSA-CA fluorescence in the mesogleal tissues (Figs. 1 and 4A). A distinct banding pattern, which was always associated with a cluster of amoebocytes con- taining zooxanthellae, was also occasionally seen in the oral epithelial layer (Fig. 4B). In field-condition and bleached animals, the oral epithelial layers had, respectively, about 27% and 32% greater DNSA-CA fluorescence than aboral bell margin epithelial layers (field-condition: P = : bleached: P = ) ( Figs. 4A, 5A. and 5B). The sum of oral and aboral epithelial labeling of field-condition jel- lyfish sections had 44% greater DNSA-CA fluorescence than the sum of oral and aboral layers from sections of bleached animals (P < ). Sectioned tissues of field-condition and bleached animals incubated in 50 ju,A/ DNSA alone had respectively 13 and 8 times more DNSA-CA fluorescence than when these tissues were incubated in 2 mM AZ + 50 juAf DNSA (P < Figure 4. DNSA-CA fluorescence in sectioned tissue of Cuxxiopea xanuichuna bell margin. Abbreviations: O. oral sur
Size: 1475px × 1694px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
