. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . he erythrocytes fresh and thoroughly washed,the saline solution isotonic and all reagents accurately diluted, hemolysisat the end of the hours incubation is complete (Plate V, e) in the firstsix tubes, nearly complete (Plate V, d) in the seventh, partial (Plate V, c)in the eighth, slight in the ninth, and lacking (Plate V, a and b) in thelast four tubes. Then since is the unit of hemolysis, , in general, used to sensitize of erythrocytes. The interpreta-tion of a hemoly
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . he erythrocytes fresh and thoroughly washed,the saline solution isotonic and all reagents accurately diluted, hemolysisat the end of the hours incubation is complete (Plate V, e) in the firstsix tubes, nearly complete (Plate V, d) in the seventh, partial (Plate V, c)in the eighth, slight in the ninth, and lacking (Plate V, a and b) in thelast four tubes. Then since is the unit of hemolysis, , in general, used to sensitize of erythrocytes. The interpreta-tion of a hemolysin titration can be learned by experience only. Hemolysin Titration. 10 per cent. complement. Number oftube. Hemolysin instandarddilution. 1 2 3 4 5 6 7 8 9 10 11 12 5 per cent. erythrocyte per cent. suspension. saline. .a 0,1 •So Sl is Sts §i Si i fl PLATE V. a. Complete absence of hemolysis before settling of cells. b. Conciplete absence of hemolysis after settling of cells. c. Partial d. Nearly complete hennolysis. e. Complete hemolysis. ANTIGEN 193 Complement Titbation. Hemolysin in 5 per cent. Number of 10 per cent. standard erythrocyte per cent. tube. complement, dilution. suspension. saline. 1 (D 2 ^ 3 to 4 So 5 o t^ ^ CO 6 §^ • 7 °:S 8 °l 9 ?7-1 ^ 10 ^1 11 it 12 3 13 A Antigen Titbation. Number of 10 per cent. per cent. SensiLizeaerythrocyte tube. Immune serum Antigen complement ;. saline. suspension. CO. CO. 1 a 2
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