. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. Vegetative Organs of Vascular Plants 137 complete or even incorrect picture of the woody stem in the mind of the student. Twigs are not satisfactory for making longitudinal sections in quantities. Unembedded twigs cannot be held in the microtome horizontally for longitudinal sections. If an embedded and blocked twig is sectioned longitudinally, only the outermost sections are strictly tangential, and only a few slices fro
. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. Vegetative Organs of Vascular Plants 137 complete or even incorrect picture of the woody stem in the mind of the student. Twigs are not satisfactory for making longitudinal sections in quantities. Unembedded twigs cannot be held in the microtome horizontally for longitudinal sections. If an embedded and blocked twig is sectioned longitudinally, only the outermost sections are strictly tangential, and only a few slices from the center are true radial sections, cut parallel to a ray. For first-class preparations cut accurately on the three desired planes, use blocks of wood and attached bark removed from living trees as illustrated in Fig. Sectioning of such blocks is quite impossible without embedding in celloidin; whereas, with the celloidin method, perfect sections can be produced in quantities (Fig. 6, c). Collect the material in the winter when the cambium is firm. Soft wood like basswood, white pine, apple, or silver maple can be cut without special softening. Kill in FAA, and embed in celloidin. Hard woods like oak or locust must be treated with hydrofluoric acid after killing and hardening in FAA. The protoplasts cannot be expected to be in perfect condition after treatment in HF. The process is described in Chap. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sass, John E. (John Eugene), 1897-1971. Ames, Iowa State College Press
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Keywords: ., bookcentury1900, booksu, booksubjectbotany, booksubjectmicroscopy
