. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HIGH PRESSURE RESPIROMETER is shown in Figure It consists of a 20-cni. length of 2 mm. bore capillary tubing, calibrated in cc. units, and fitted by a 20/40 standard taper joint to a 30-cc. Pyrex shell vial, 85 mm. long. To the tip of the capillary is fused a cm. length of 7-mm. bore Pyrex tubing. The latter is slightly constricted at its distal open end and serves as a reservoir for the index drop solution. The calibrated volume of each capillary is 550 The total volume of each volumeter to the tip of the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HIGH PRESSURE RESPIROMETER is shown in Figure It consists of a 20-cni. length of 2 mm. bore capillary tubing, calibrated in cc. units, and fitted by a 20/40 standard taper joint to a 30-cc. Pyrex shell vial, 85 mm. long. To the tip of the capillary is fused a cm. length of 7-mm. bore Pyrex tubing. The latter is slightly constricted at its distal open end and serves as a reservoir for the index drop solution. The calibrated volume of each capillary is 550 The total volume of each volumeter to the tip of the capillary is 45 FIGURE 3. A 45-cc. capillary volume'.er. The respiration chamber can be attached to a standard Warburg manometer by means of an adapter previously described (Schneiderman and Williams, 1953). Thus the respiration of the animal in air before and after exposure to high pressure may be conveniently measured by conventional methods without removal of the ani- mal from the respiration chamber. c. Capillary barometer. Measurements of absolute pressure, accurate to within one per cent, are required for the proper determination of gas exchange in the present apparatus (see discussion of calculations below). Since the standard Bour- don type pressure gauges are subject to errors in excess of 5 per cent, a capillary barometer is utilized. Each such barometer consists of a one-cc. pipette of approxi- mately 2 mm. internal diameter, graduated in cc., and sealed at one end. By means of a long hypodermic needle a drop of colored detergent solution is placed in the closed end of the capillary and another drop at the beginning of the gradu- ations. In each experiment three capillary barometers of this type are enclosed in the Lucite compensation chamber. The pressure in the closed system is calculated by application of the gas law from readings of the capillary barometer taken before and after compression and from a reading of the local barometric pressure. d. Water
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
