. Gaseous exchanges in isolated muscle and the perfection of an apparatus for studying same . 49, to absorption chamber II. Correction for atnos-pheric CO2 in first period estimation. Temperature 19* : Hours from GO2 GO2 rate Air current Time start obtained per hr rate per hr 8:49- 9:19 .286 cc .286 cc liters 9 :19- 9 :49 1 .164 .164 7 .6 (Estima- 2 .276 .138 7 .6 tions 3 .226 .113 8 .6 hourly 4 .216 .108 until 5 .190 .095 9 .0 10:49 ) .200 . 100 8 .8 7 . 240 .120 7 6 ft .200 .100 8 .2 9 .200 .100 10 .200 .100 6 .6 11 .172 .086 6 .3 12 .176 .088 7 .8 13 .17


. Gaseous exchanges in isolated muscle and the perfection of an apparatus for studying same . 49, to absorption chamber II. Correction for atnos-pheric CO2 in first period estimation. Temperature 19* : Hours from GO2 GO2 rate Air current Time start obtained per hr rate per hr 8:49- 9:19 .286 cc .286 cc liters 9 :19- 9 :49 1 .164 .164 7 .6 (Estima- 2 .276 .138 7 .6 tions 3 .226 .113 8 .6 hourly 4 .216 .108 until 5 .190 .095 9 .0 10:49 ) .200 . 100 8 .8 7 . 240 .120 7 6 ft .200 .100 8 .2 9 .200 .100 10 .200 .100 6 .6 11 .172 .086 6 .3 12 .176 .088 7 .8 13 .170 .085 7 .4 (Left to run 14 .184 .092 8 .4 over night until 8:49 15-24 2 .456 ( .125) ( . passed ) through, but going next A 6 :49- 9 :49 25 .810 .405 9 :49-10 :49 26 .860 .430 7 .6 Legs had a distinct putrefactive s;iioil on removal. Quite rigidB»ol:;t. Putrefaction p:ol)ably began v/ith increased output of 14th hour .3t .lb - 80 - rire of CO^ ohtdnneci jjcx^-^lir. from fro^^ le^s rest ^eoThng/es ~ \ro/vn^e^ of CO^ per period Mt^/vJec/Red lines— r«a7e of /fir Cvrrent. 16 II IZ. O c 5 10 g. b A2 /3 74 Experiment 7 To Determine: Amount and rate of CO2 evolution from both legsof frog at rest. Method: Iedium sized frog killed at 8:37. Both legs skinned,severed at pelvis, (with as little injury to thigh muscles as pos-sible) and at ankles. Placed in chamber A at 8:53 and currentstarted at once to absorption chamber I. Atmospheric CO^ in chambercorrected for in first period estimation. Data:Time Hours from start CO2 obtained CO2 per hr Air currentrate^jpe^r Jir Tompeatu_re^ 8:53- 9:23 .286 cc .286 cc liters 23 C 9:23- 9:53 1 .206 .206 10 .0 23 (Estima- 2 .238 .169 23 tions 3 .300 .150 23 nou 4 .280 .140 24 until 5 .262 .141 9 .5 24 10:53 ) 6 .246 .123 - 8 .2 7 .268 .134 7 .1 23 .5 8 .258 .129 7 .7 23 .5 9 .286 .143 7 .8 24 lC-11 .586 .146 6 .4 24 12 .304 .152 6 .8 24 .5 13 .304 .152 8 .4 24 .5 P .M. 10:53-11-23 14 14 .5 .35


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