. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. ZINC AND COPPER LEVELS FOR STEELHEAD TROUT 85 packed with spun glass for removal of residual ferric oxide and hydroxide precipitate. The spun glass was replaced weekly. After filtration, the prediluted waste then flowed into the toxicant cells of the proportional diluters. PMDIIUTON CHAMIEK Solenoid Valve OIIUTION WATE> LEGEND \0 ' ^ To Drain DILUTION WATER CELLS. ~i r~ REPLICATE BIOASSAY TROUGHS PEDISTALIIC PUMF F


. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. ZINC AND COPPER LEVELS FOR STEELHEAD TROUT 85 packed with spun glass for removal of residual ferric oxide and hydroxide precipitate. The spun glass was replaced weekly. After filtration, the prediluted waste then flowed into the toxicant cells of the proportional diluters. PMDIIUTON CHAMIEK Solenoid Valve OIIUTION WATE> LEGEND \0 ' ^ To Drain DILUTION WATER CELLS. ~i r~ REPLICATE BIOASSAY TROUGHS PEDISTALIIC PUMF FIGURE 2. Schematic diagram of waste predilution system and modified Mount and Brungs type proportional diluter. The waste concentrations and control dilution water from the proportional diluters were divided in two and supplied to replicate plexiglass bioassay troughs. The troughs were 92-cm long by 13-cm wide, with the water level adjusted to a depth of 10 cm by a standpipe. The troughs were subdivided into five compartments by alternating baffles which allowed for the complete circula- tion of the diluted wastes over the developing eggs, alevins, and fry. The fertil- ized eggs were incubated in egg baskets, three baskets per trough. The egg baskets were perforated polyethylene plastic jars, in diameter and 9-cm high. The troughs had a volume of approximately 12 liters, and approximately 500 ml of the diluted waste was supplied to each trough every 7 minutes. The volume of each trough was replenished every hours. Continual-flow bioassays using standard methods (Peltier 1977) were carried out at WPCL with freshly fertilized steelhead trout eggs and the resulting alevins and swim-up fry. All concentrations were tested in replicate. The eggs were obtained by dry spawning three female and two male adult fish on 10 February 1978 at the Department's Nimbus Hatchery on the American River. Following dry spawning, the eggs were segregated and placed in the different concent


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