. Diseases of bees. Bees. FUNGOUS DISEASES OF THE HONEYBEE 27 The fungous fluids were pressed from the mycelium after" it had been â washed and ground with sand. Both tlie medium and the fungous fluids were each shaken five times with small volumes of chloroform and separated with a separatory funnel after each operation. After washing in an evaporating dish the chloroform was evaporated to dryness, and the residue from each solution was taken up with about 5 cubic centimeters of distilled water and given to worker bees with ah equal volume of honey. In each case the entire quantity of fo
. Diseases of bees. Bees. FUNGOUS DISEASES OF THE HONEYBEE 27 The fungous fluids were pressed from the mycelium after" it had been â washed and ground with sand. Both tlie medium and the fungous fluids were each shaken five times with small volumes of chloroform and separated with a separatory funnel after each operation. After washing in an evaporating dish the chloroform was evaporated to dryness, and the residue from each solution was taken up with about 5 cubic centimeters of distilled water and given to worker bees with ah equal volume of honey. In each case the entire quantity of food was consumed without evidence of poisoning. Kepetitions of this experiment at different stages in the development of the lungus gave entirely negative results. It would seem therefore that bases capable of poisoning bees when administered with food are not produced by A. -flavus Ao5c. Chemical Nature of the Toxio Sitbstancb In most cases chemists have been unable to determine the constitu- tion of toxic substances elaborated by microorganisms; therefore only ^ ,, /srreML I*" ^ so \ *" \ 30 \ ^o S o -^ / / / Zf/p rs/^^. /?^ys PrGUEir 2.âRate of mortality of honeybees at dally intervals after the commencemexit of feeding with honey containing ether extract of the nutrient miedium in which Aspergillus flavus had' been cultured. First trial made immediately after the extract was prepared, second trial 15 daya after the extract was prepared. a few tests were made to determine the general nature of this sub- stance. Tests for phenolic compounds were floating small quantities of freshly prepared ether extract on very dilute ferric chloride solution. The absence of color reaction in all cases indicated the absence of such compounds in the extract. When the extract from flask cultures in 5 cubic centimeters of water was mixed with 5 cubic centimeters of honey, the resulting solution showed a hydro- gen-ion concentration of about pH by the colorimetric method o
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