. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Dissection and perfusion technique. A. Gross dissection. The mantle was cut up the midline and reflected to reveal the organs in the mantle cavity. B. Enlarged region denoted by dotted lines in A after removal of the syphon, the ventral gut musculature, and the digestive gland. C. As in B but after removal of the vein, trimming of the remaining gut muscle to reveal the ganglia, and cannulation of the aorta. M, mantle; IS, ink sack; GI, gill; G, digestive gland; SY, syphon; V, vein; SN, stellar nerve; SG, stellate g
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Dissection and perfusion technique. A. Gross dissection. The mantle was cut up the midline and reflected to reveal the organs in the mantle cavity. B. Enlarged region denoted by dotted lines in A after removal of the syphon, the ventral gut musculature, and the digestive gland. C. As in B but after removal of the vein, trimming of the remaining gut muscle to reveal the ganglia, and cannulation of the aorta. M, mantle; IS, ink sack; GI, gill; G, digestive gland; SY, syphon; V, vein; SN, stellar nerve; SG, stellate ganglion; E, point of descent of the prenerve through the digestive gland muscle wall; T, tubing; PO, medial stellar nerve—containing the largest postsynaptic giant axon; CA, point of exit and descent of the common stellate artery from the aorta; GM, cut edge of the digestive gland muscle wall; L, ligature; PR, pre-nerve— containing the presynaptic giant axon; C, cannula; SA, stellate artery. second, larger and wrinkled vessel is a vein which can be carefully stripped off. The artery supplies both ganglia from a single branch (which we term the common stellate artery) that passes through the underlying muscle and bifurcates to the ganglia (the right and left stellate arteries). After passing the ganglia, the stellate arteries also supply the rostral third of the mantle musculature. Perfusion of the ganglia requires several ligatures (Fig. Ic). One ganglion, the right, was selected for the present study. The medial aorta was tied off with 6-0 suture just rostral to the common stellate branch. Ligatures were tied loosely at two points on the aorta, caudal to the common stellate artery, in readiness for the cannula. The rostral ligature of this pair was positioned very closely to the common stellate artery. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations
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