. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilllermond - Atkinson 180 Cytoplasm off and forms the small vacuole of the daughter cell. Dangeard has sought more recently to demonstrate, but this time with vital dyes, that the vacuoles of algal zoospores are always transmitted by means of the filament put out at germination. In Saprolegnia, growing on media to which neutral red has been added and observed in van Tieghem and Le Monnier cells, we have shown, however, that the vacuoles which in the zoospores appear as small granules, fuse at the moment of germination to
. The cytoplasm of the plant cell. Plant cells and tissues; Protoplasm. Guilllermond - Atkinson 180 Cytoplasm off and forms the small vacuole of the daughter cell. Dangeard has sought more recently to demonstrate, but this time with vital dyes, that the vacuoles of algal zoospores are always transmitted by means of the filament put out at germination. In Saprolegnia, growing on media to which neutral red has been added and observed in van Tieghem and Le Monnier cells, we have shown, however, that the vacuoles which in the zoospores appear as small granules, fuse at the moment of germination to constitute a single large vacuole and then, in the germination tube, small globular vacuoles appear which do not seem to be derived from the large vacuole of the zoospore. Now, if the objection of Pierre Dangeard is sound in regard to vital staining of the ordinary fungi which, when carried out between slide and cover. * > a ⢠â ⢠7 ⢠A * a J ⢠1 1 Fig. 125 (left). â Saprolegnia. Germination of zoospores in a van Tieghem and Le Monnier cell on 1% peptone bouillon with neutral red. 1, 2, zoospore. 3-20, germination tube. The large vacuole extends into the germina- tion tube and may fuse (15) with small vacuoles which form at the tip. (After Mile. Cassaigne) . Fig. 126 (right). - vacuoles during budding cell without vital dyes, mentation and fusion. vacuole of mother cell, formed by a kind of Cassaigne) . - Saccharomyces pastorianus. Types of formation of of the yeast grown in a van Tieghem and Le Monnier A, 1-8, Observation during 1 hr. 1-5, successive frag- 6, 7, prolongation sent into bud. 8, separation from B, vacuole of bud formed de novo. C, vacuole of bud budding from vacuole of mother cell. (After Mile. glass do not grow as long as they keep the neutral red accumulated in their vacuoles, this objection is evidently not sound in the case of Saprolegnia, which can be grown on media to which neutral red has been added. Mile. Cassaigne repeated this s
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