. Ciba Foundation colloquia on ageing. Old age; Aging; Animals -- growth & development. 218 J. E. Lovelock dissolution of red cells during their storage at low tempera- tures. The progress of the dissolution is described and the structure and state of the cell membrane discussed in the light of the experimental observations. The possible relevance of these observations to the problem of senescence is also discussed. The dissolution of red cells in the temperature range 0° to +40° The suspension of human red cells in 0-16M-NaCl is fol- lowed by a loss of lipid components from the cell membr
. Ciba Foundation colloquia on ageing. Old age; Aging; Animals -- growth & development. 218 J. E. Lovelock dissolution of red cells during their storage at low tempera- tures. The progress of the dissolution is described and the structure and state of the cell membrane discussed in the light of the experimental observations. The possible relevance of these observations to the problem of senescence is also discussed. The dissolution of red cells in the temperature range 0° to +40° The suspension of human red cells in 0-16M-NaCl is fol- lowed by a loss of lipid components from the cell membrane and to a lesser extent by haemolysis (Lovelock, 1954). The loss of lipids is rapid at first, but after 3 minutes an equilibrium level is reached, and the rate of loss falls to a low value. The equilibrium concentration of lipids does not appear to repre- sent a simple saturation of the medium with these substances, 30r. 5 10 15 NUMBER OF WASHINGS 20 Fig. 1. The removal of components from the red cell by repeated washing. The cells were suspended in 0 16 M-NaCl for 10 min. at 37°, separated by centrifuging and resuspended in fresh 0-16 M-NaCl. This was repeated 20 times. + Lipoprotein; ? Volume; 3 Cholesterol; X Phospholipid; O Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenholme, G. E. W. (Gordon Ethelbert Ward). Boston, Little, Brown
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