. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. WOLFFIIUGEL'S APPARATUS 125 sterile condition, only containing two or three germs j)er cubic centimeter. Examination of water.âFor purposes of examination I to 1 is taken with a sterilised pipette and mixed with sterile melted gelatine, which is then poured upon a plate, and the development of the colonies carried on at the temperature of the room. The number of islets formed is then ascertained with the aid of a counting apparatus, and in this way the relative value in m
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. WOLFFIIUGEL'S APPARATUS 125 sterile condition, only containing two or three germs j)er cubic centimeter. Examination of water.âFor purposes of examination I to 1 is taken with a sterilised pipette and mixed with sterile melted gelatine, which is then poured upon a plate, and the development of the colonies carried on at the temperature of the room. The number of islets formed is then ascertained with the aid of a counting apparatus, and in this way the relative value in micro-organisms of various samples of water is â Supporting slab Glass plate engi-aved in diiasions s^ Frame Fig. 45.ââ Wolffhugel'k Cousting-Plate. The counting apparatus (WolfEhiigel's counting-plate, fig. 45) consists of a black slab upon which the plate with the gelatine culture is laid, and over this is arranged a pane of glass on which squares of uniform size have been engraved. The islets in the individual squares are then counted with the help of a lens, and an average struck, when the number so obtained multiplied by the total number of squares on the plate gives approximately the total number of colonies for a certain area, a number which varies with different kinds of water. The water to be used in this experiment must not be kept, but must be examined imme- diately after collection. In exataining water presumably rich in germsâfor example, that from rivers or pondsâthe. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Schenk, Samuel Leopold, 1840-1902. London, Longmans, Green
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