. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 232 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS longitudinal view end-on view. F igure 2A. Epifluorescent intake of immunolabeled K' channel* in an inlraeellnlarls perfused sc/uid giant a\on. Without the central a\o/>lasinic con: the unsupported a\«nal membrane folded and collapsed as it \\-as mounted mi the slide Hop panel). The bottom pan of the image lin focus) illustrates paiches «/ immunqfluorescence from Mm membrane surface's. I'hc 1,1/1 purl «/ the image loin of locus) .showed .sunning similar to thai in the h
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 232 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS longitudinal view end-on view. F igure 2A. Epifluorescent intake of immunolabeled K' channel* in an inlraeellnlarls perfused sc/uid giant a\on. Without the central a\o/>lasinic con: the unsupported a\«nal membrane folded and collapsed as it \\-as mounted mi the slide Hop panel). The bottom pan of the image lin focus) illustrates paiches «/ immunqfluorescence from Mm membrane surface's. I'hc 1,1/1 purl «/ the image loin of locus) .showed .sunning similar to thai in the hottoni part of the inia,gi- when it mis brought into focus. B. Epiflno- rescenl linage of an a\on idi/tcrcnl than the one described in A) perfused \\ilh a K channel antihoilv that does not c\l. The bar below this image represents /fill (same lor A ami K). dittuse signal throughout the membrane—not present in the control axons—in addition to the patches of intense immuno- fluorescence shown in Figure 2A. Preliminary results with immunogold suggest that the immunofluoreseence is attribut- able to K+ channels in the membrane rather than to K + channel containing vesicles which have not been removed dur- ing extrusion of the axoplasm. Our results provide further support to the argument that SqKvlA mediates the classic delayed rectifier potassium ion current in the squid giant axon (4). Punctate ion-channel immunofluoreseence has been observed in a number of preparations. The results most relevant to this study are those of Johnson et ul. (8). They demonstrated, in cultured Aplysin axons. clusters of Na+ channels that were separated by regions where channels were not present, similar to nodes of Ranvier in myelinated axons (9). Our results are consistent with the punctate nature of immunofluoreseence observed in Aplysin axons, but the patches of fluorescence in squid axons appear to be uniform throughout. We speculate that the membrane patches of ion chann
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
