. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 348 FERTILIZATION AND DEVELOPMENT 4. Segal, S. J., and Ueno. H. 1989. Biol. Bull 177: 316. 5. Metzler, C. M., et al. 1980. / Am. Client. Soc. 102: 6075-6082. 8. 6. Segal, S. J., et al. 1985. Biol. Bull. 169: 543-544. 9. Burgos, M. H., et al. 1980. Bn>l. Bull 159: 467. Kanwar, U., et al. 1989. Contraception 39: 431-445. Ueno, H., et al. 1987. Biol. Bull 173: 428. KCl-Induced Calcium Rise in Squid Eggs: Measurement of Fura-2 Fluorescence Rui Wang, Lingyun Wit, Andrew L. Miller, John M. Arnold, and Lionel F. Jaffe (Marine Bi
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 348 FERTILIZATION AND DEVELOPMENT 4. Segal, S. J., and Ueno. H. 1989. Biol. Bull 177: 316. 5. Metzler, C. M., et al. 1980. / Am. Client. Soc. 102: 6075-6082. 8. 6. Segal, S. J., et al. 1985. Biol. Bull. 169: 543-544. 9. Burgos, M. H., et al. 1980. Bn>l. Bull 159: 467. Kanwar, U., et al. 1989. Contraception 39: 431-445. Ueno, H., et al. 1987. Biol. Bull 173: 428. KCl-Induced Calcium Rise in Squid Eggs: Measurement of Fura-2 Fluorescence Rui Wang, Lingyun Wit, Andrew L. Miller, John M. Arnold, and Lionel F. Jaffe (Marine Biological Laboratory) Sperm break the block to development in unfertilized eggs by somehow inducing a large increase in intracellular free Ca2+ ([Ca2+],). The mechanisms and sources for the increase in [Ca2+], have been hypothesized to be different in deuterostome and pro- tostome eggs (1). A release of intracellular Ca2+ upon fertilization plays a major role in the regenerative and explosive increase in [Ca2+], in deuterostome eggs. By contrast, extracellular Ca2+ entry may be more important for the prolonged increase in [Ca2+]j in protostome eggs. However, solid evidence substantiating this hypothesis in protostome eggs is limited. Squid eggs are unique in their size, mode of development, and phylogenetic position among protostomes (2). We have used these eggs to 1) investigate the existence of voltage-dependent calcium channels in unfer- tilized egg membranes, 2) establish the methodology of the fura- 2 fluorescence measurement, and 3) correlate KCl-induced Ca:f rise with egg activation. Squid (Loligopealef) were provided by the Marine Resources Department of the Marine Biological Laboratory during July and August. Clear, mature eggs were collected from the oviduct of female squid and washed several times with aerated millipore filtered artificial seawater (ASW). These eggs were put on cov- erslips, coated with poly-1-lysine. and maintained in ASW. A dual-excitation photomete
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