. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 132 B. BAILEY, P. KORAN AND H. C. BRADLEY ten-day period about 30 per cent of the total muscle protein had digested. Added hemoglobin is nearly completely digested in the time allotted to the experiment. Digestion was still in progress at the end of ten days, and somewhat more of the muscle proteins would have undergone cleavage if the time had been extended. .301 \O 2O 60 70. FIGURE I. The curves indicate cleavage as measured by the tyrosine color, at- tained by a series of digests in 24 hours where pH level
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 132 B. BAILEY, P. KORAN AND H. C. BRADLEY ten-day period about 30 per cent of the total muscle protein had digested. Added hemoglobin is nearly completely digested in the time allotted to the experiment. Digestion was still in progress at the end of ten days, and somewhat more of the muscle proteins would have undergone cleavage if the time had been extended. .301 \O 2O 60 70. FIGURE I. The curves indicate cleavage as measured by the tyrosine color, at- tained by a series of digests in 24 hours where pH levels were set and maintained. Three- and five-day curves are omitted. Tissue alone is represented by solid lines; tissue and hemoglobin by dotted lines. The vertical distance between the two curves of the same duration represents the amount of tyrosine referable to the added substrate hemoglobin. In this muscle it is evident that half of the total tyrosine liberated in 24 hours came from hemoglobin cleavage. Autolysis of carp muscle may be compared with that of albacore and Pacific mackerel under identical conditions, Figures II and III. It will be noted that relatively more hemoglobin digests the first day in carp muscle autolysis than in the autolysis of mackerel or albacore muscle. On the other hand there is much more of the active mackerel and albacore muscle protein digested in one day than in the case of the carp. This difference is summarized in Table II. Assuming that the tissue proteinase distributes itself between the muscle proteins and the hemoglobin in proportion to the relative masses of the two proteins, then it would appear that carp muscle is less easily fragmented than hemoglobin, while mackerel and albacore proteins are more easily fragmented by the tissue proteinase. The data suggest that the muscle proteins of the active fish are more fragile to the enzymes. Please note that these images are extracted from scanned page images that may have been digitally enhan
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
