. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 394 HERBERT SCHUEL in the interior of the cell (Heilbrunn, 1956). This too proved to be unsuccessful (Fig. 1). In the presence of higher concentrations of potassium ions, the eggs did not divide at all. Histamine and histidine, on the other hand, did accelerate recovery. The results of a typical experiment can be seen in Figure 3. The possible role of proteolytic enzymes in cellular sol-gel transformations has already been discussed. The addition of small quantities of chymotrypsin 100 - 80 - LJ O < < LJ _J O 60 - 40 -
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 394 HERBERT SCHUEL in the interior of the cell (Heilbrunn, 1956). This too proved to be unsuccessful (Fig. 1). In the presence of higher concentrations of potassium ions, the eggs did not divide at all. Histamine and histidine, on the other hand, did accelerate recovery. The results of a typical experiment can be seen in Figure 3. The possible role of proteolytic enzymes in cellular sol-gel transformations has already been discussed. The addition of small quantities of chymotrypsin 100 - 80 - LJ O < < LJ _J O 60 - 40 - 20 - 0 control 1 = 1% urethan o o " + histamine -h- histidine 10"4%. 0 i 2 i 3 \ 4 i 5 \ 6 HOURS AFTER INSEMINATION FIGURE 3. Attempt to overcome urethan inhibition of division by means of histamine and histidine. Eggs were exposed to urethan-containing solutions 5 minutes after insemination. ("Worthington 3X," crystals) or of trypsin ("Worthington lyophilized," crystals) accelerated the recovery of the eggs inhibited by 1% urethan (Fig. 4). Since calcium is believed to be bound to lipoprotein in the cortex of the cell (Heilbrunn, 1956), it was thought that lipase might also tend to relieve the urethan inhibition. Wheat germ lipase (Worthington) proved to be effective (Fig. 4). In one experiment the eggs were incubated in a mixture of trypsin and lipase ( ml. each). The results were identical with those obtained by trypsin alone. How- ever, after 24 hours the eggs were almost completely digested in the trypsin-lipase mixture, while they remained intact when incubated with either of these enzymes. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-19
Size: 1273px × 1963px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
