. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 16 KAMIO ET Spiking response B *»-y\Hr—-^-v^HMr^^r^Mr 1 M!" Spontaneous response Odor-evoked response 10ms Figure 3. Responses of chemosensory neurons in the antennular outer flagellum of a male specimen of Telmessus cheimgomis to shrimp extract. (A) Extracellular recording from antennular nerve to the injection of shrimp extract (lower trace) and its integrated activity (upper trace). Time of injection is indicated by arrow. (B) Spike activity of the antennular nerve before injection of shrimp extract. (C) Spike a


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 16 KAMIO ET Spiking response B *»-y\Hr—-^-v^HMr^^r^Mr 1 M!" Spontaneous response Odor-evoked response 10ms Figure 3. Responses of chemosensory neurons in the antennular outer flagellum of a male specimen of Telmessus cheimgomis to shrimp extract. (A) Extracellular recording from antennular nerve to the injection of shrimp extract (lower trace) and its integrated activity (upper trace). Time of injection is indicated by arrow. (B) Spike activity of the antennular nerve before injection of shrimp extract. (C) Spike activity of the antennular nerve in response to injection of shrimp extract. (B) and (C) correspond to part "B" and "C" of the lower trace of Figure 3A. chemoreceptors on the outer flagellum to both female and male urines, the responses of outer flagellum afferents to various concentrations of female and male urine were ana- lyzed quantitatively by normalizing them to the response to 1 mM L-serine (standard reference stimulus: STD) (Fig. 5). The outer flagellum showed larger responses to female urine than to male urine at all dilutions, and the differences were significant at dilutions of 10~3 (z = , P < : Wil- coxon matched pairs test) and 10~4 (- = , P < ). Overall differences among ASW, female urine, and male urine were also significant (Friedman ANOVA, P < ). Ami/id and analysis Male and female urines contained detectable amounts of common amino acids and nitrogenous compounds; taurine. urea, and ammonia were most abundant in the urine, rang- ing from 49 to 248 , while the rest of the compounds were present at less than 20 Concentrations of com- pounds were higher in male urine than in female urine, except for ethanolamine, -y-aminobutyric acid, and phos- phoserine (Fig. 6). Overall, concentration differences of the chemicals between male and female urines were significant (Wilcoxon matched pairs test. - = , P


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology