. ONTARIO SESSIONAL PAPERS, 1914, the glassware frequently still hot they wereplaced in the bottles by means of a pair of sterilized tongs. Dilutions of the sample of sewage were obtained by pipetting 1 or 10 cc. of thesample into a dilution bottle containing 100 cc. with the added quantity. This gavea dilution of 1 in 100 or 1 in 10 as required, greater dilutions were made by follow-ing the same procedure and taking 1 cc. from the first dilution bottle and placingin a second and so on. A regular technique of 50 shakes was employed for both samples and dilutions through the entire wo


. ONTARIO SESSIONAL PAPERS, 1914, the glassware frequently still hot they wereplaced in the bottles by means of a pair of sterilized tongs. Dilutions of the sample of sewage were obtained by pipetting 1 or 10 cc. of thesample into a dilution bottle containing 100 cc. with the added quantity. This gavea dilution of 1 in 100 or 1 in 10 as required, greater dilutions were made by follow-ing the same procedure and taking 1 cc. from the first dilution bottle and placingin a second and so on. A regular technique of 50 shakes was employed for both samples and dilutions through the entire work. J* (b) Washing and Sterilizing Petri Dishes. In all about 200 Petri dishes were used each day at the Experimental Station,and it is readily seen that washing and sterilizing them involved a considerableamount of time and labour. However, a technique was finally hit upon by which200 plates could be washed and placed in copper boxes ready for sterilizing in 30minutes. The dirty plates were placed on the right-hand side of the sink while.


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