. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 24 Marine Invertebrates A few organisms may swim back through the siphon connecting the second and the first jar and be lost. The opening of the controlling siphon may be covered with plankton silk or other fine material to pre- vent the escape of minute organisms. This arrangement has been suc- cessfully used by the author in rearing and maintaining small crusta- ceans, the larvae of mollusks and echinoderms, and small jelly fishes. Further improvement of thi
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 24 Marine Invertebrates A few organisms may swim back through the siphon connecting the second and the first jar and be lost. The opening of the controlling siphon may be covered with plankton silk or other fine material to pre- vent the escape of minute organisms. This arrangement has been suc- cessfully used by the author in rearing and maintaining small crusta- ceans, the larvae of mollusks and echinoderms, and small jelly fishes. Further improvement of this method was made by F. G. Walton Smith whose personal communication reads as follows: "Rapid clog- ging of the pores of this material (bolting silk) is prevented by dipping the mouth of the siphon under the surface of molten paraffin wax melt- ing at 48° C. and then blowing air through the other end as it is removed. The resulting smooth coating of wax on the fibers seems to prevent. Fig. 29.—The current rotor. the entanglement of larvae and allows the filter to work efficiently for a much longer period than would otherwise be the case. The net is attached to the siphon by means of a wide rubber band, and is of such a nature that when worked, the openings are just small enough to serve to retain the ; Using this technique Dr. Smith had no difficulty in growing oyster larvae at the U. S. Bureau of Fisheries Station at Beaufort, N. C. Slow exchange of water may be obtained by using the so-called "filtros" block which may be installed as a partition in the aquarium, or by inserting a Berkefeld filter and slowly sucking the water through it. Stone filters become clogged very quickly and require frequent changes. Current rotor. This instrument is designed to change the water in the aquarium without losing the small organisms living in it. The es- sential feature of the apparatus (Fig. 29) designed by Galtsoff and Cable (1933) is a cylinder (A) of 60 me
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