The Journal of biological chemistry . ali reserve which changed either thepKi of the serum or changed the constancy of relation of thecolorimetric determination to the electrometric determinationof hydrogen ion concentration. In Experiment 18 it is evident that the pKi of the serum wasthe same before and after anesthesia. In Experiments 19 and 20it is evident that the decrease in pH as measured electrometicallyis practically identical with the decrease in pH as determinedcolorimetrically. These results indicate the accuracy of thecolorimetric pH measurement in experiments of this nature. Group
The Journal of biological chemistry . ali reserve which changed either thepKi of the serum or changed the constancy of relation of thecolorimetric determination to the electrometric determinationof hydrogen ion concentration. In Experiment 18 it is evident that the pKi of the serum wasthe same before and after anesthesia. In Experiments 19 and 20it is evident that the decrease in pH as measured electrometicallyis practically identical with the decrease in pH as determinedcolorimetrically. These results indicate the accuracy of thecolorimetric pH measurement in experiments of this nature. Group I.(Experirnents 1, 2, S, 4, 5, and 17; Figs. 1 and 2.) In the experiments of this group it will be observed that bothwith ether and with chloroform an extremely rapid fall in reserve of from 4 to 11 mM. occurs in the first few is associated with rise of CO2 tension and fall of pH. As theanesthesia proceeds the fall in alkali reserve continues, but atcontinually decreasing rate. The CO2 tension tends to return. 632 CuUen, Austin, Kornblum, and Robinson 633 toward normal in consequence in part no doubt of the increasingventilation rate which we reported in the previous study. Syn-chronously, either the rate of fall in pH diminishes or there maybe a return toward normal pH. After cessation of ether anesthesia Changes ^ ^ o u O- Exoeriment 17 Ether with p^cov^PY
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiochem, bookyear1905
