. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 220 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 1. Aplysui neuronal growth cone. : effect of c\tochalasin B on the growth cone. (.4) Pol-Scope image of the growth cone before application nf cytochalasin B, (B) after application of cvtochuliisin B. C: Rhodamine-phalloidine stain of the growth cone demonstrating F-actin. D. E: Comparison of Pol-Scope image with DIC image. (D) Pol-Scope image. (E) DIC image. Pol-Scope images show the measured retardance magnitude in similes of gray (black is :ero. while I nm ret
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 220 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 1. Aplysui neuronal growth cone. : effect of c\tochalasin B on the growth cone. (.4) Pol-Scope image of the growth cone before application nf cytochalasin B, (B) after application of cvtochuliisin B. C: Rhodamine-phalloidine stain of the growth cone demonstrating F-actin. D. E: Comparison of Pol-Scope image with DIC image. (D) Pol-Scope image. (E) DIC image. Pol-Scope images show the measured retardance magnitude in similes of gray (black is :ero. while I nm retardance). field of view. This is a significant difference between the Pol- Scope and the traditional polarized-light microscope. These qualities achieved through optical and computational image pro- cessing give the Pol-Scope an advantage compared with DIC (See Fig. ID. E). in revealing molecular fine structure such as thin actin bundles. Furthermore. Pol-Scope images can be interpreted quantitatively because they are measurements of ob- ject retardance at each image point. On the other hand, DIC achieves image contrast through optical processing alone, allowing one to see a live image through the eye piece. Also, DIC more sensitively can detect spatial changes in refractive index or in optical path length. Therefore, both methods are often complementary and have advantages in specific applica- tions. In this report, we have demonstrated that the Pol-Scope can reveal actin-based structures in the growth cone. We are cur- rently analyzing the quantitative results provided by the Pol- Scope with regard to the actin dynamics in living growth cones. This work is supported in part by NIH grants GM49210 awarded to RO, P41RR01395 to PJSS, and NSF grant MCB 9506279 to GL. thanks Ms. A. DePina for her invaluable suggestions. Literature Cited 1. Forscher, P., and S. .1. Smith. 1988. J. Cell. Biol. 107: 1505- 1516. 2. A., and T. J. Mitchison. 1991. Nature 352: 126-131. 3.
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