Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . Fig. 139.—Preparation of blood smear with cigarette paper. {Da Costa.) cleaning and later use of the slides. The beginner may find, on examining hisearly specimens made by this method, that the leucocytes collect at the distalend of the smear and that the general surface contains few white cells. Thisis due to the use of undue pressure in making the smear, and may be avoidedby proper attention to this detail. A second method which has many advocates is the use of two cover-glasses. One
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . Fig. 139.—Preparation of blood smear with cigarette paper. {Da Costa.) cleaning and later use of the slides. The beginner may find, on examining hisearly specimens made by this method, that the leucocytes collect at the distalend of the smear and that the general surface contains few white cells. Thisis due to the use of undue pressure in making the smear, and may be avoidedby proper attention to this detail. A second method which has many advocates is the use of two cover-glasses. One clean, dry cover-glass, which should not be too large (preferablythree-fourths inch square or the larger rectangular slips), is held by the Ehrlichor cross-bladed forceps or, as some advise, between the thumb and first fingerof the left hand. The other cover, held in the pinch forceps or between thethumb and first finger of the right hand, is touched to the drop of blood as it wellsfrom the puncture in the ear. This second cover is then dropped at once upon. Fig. 140.—Ehrlich forceps the first in such a way that the corners of the two glasses do not coincide. Ifthe glasses are clean the blood spreads out evenly in a thin capillary layer be-tween them. As soon as the spreading is complete, the two covers are drawnapart, in a line parallel to the plane of their surfaces, by a steady, quick motion,being sure to avoid lifting them apart. This manipulation can be learnedonly by practice and never from any description. If the fingers are dry andif care be taken to touch only the corners of the covers the forceps need notbe used in separating the covers, but it must be remembered that moisture willcause changes in the specimen. It is, therefore, advisable to use the forcepsin this part of the technic unless the rectangular sHps be used. As soonas the covers are separated, they are allowed to dry in the air or by wavingthem two or three times to and fro. They should be at once pla
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