. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 8 C. A. BARTBERGER AND S. K. PIERCE, JR. c 0) ^_^ 1 1 c _o i i i ^ E >3 < 5!0 mOsm • 1020 mOsm - 0 4 12 24 36 Time (hr) 48 60 FIGURE 3. Time course of changes in hemolymph ammonia concentrations of propped-open M. deinissits during low salinity acclimation. Mussels were previously acclimated to full strength sea water. Points are mean values from twelve animals. Error bars designate standard error. (Fig. 3). After 24 hours, however, experimental animals had a significantly higher concentrat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 8 C. A. BARTBERGER AND S. K. PIERCE, JR. c 0) ^_^ 1 1 c _o i i i ^ E >3 < 5!0 mOsm • 1020 mOsm - 0 4 12 24 36 Time (hr) 48 60 FIGURE 3. Time course of changes in hemolymph ammonia concentrations of propped-open M. deinissits during low salinity acclimation. Mussels were previously acclimated to full strength sea water. Points are mean values from twelve animals. Error bars designate standard error. (Fig. 3). After 24 hours, however, experimental animals had a significantly higher concentration of ammonia in the blood than did the control animals (P < ), reaching a maximum concentration of /AM NH:rN/ml blood. Analysis of 60- hour samples showed that ammonia concentrations in the experimental animals were returning toward control levels by this time but were still significantly higher (P h dnrincj and following acclimation Urea was not found in the hemolymph of M. ticinissiis. Amino acid analyses of unaltered blood, a mixture of blood and urease and a mixture of blood, urea and urease resulted in identical chromatograms of the compound eluting at the >:ime time as urea in our standard amino acid mixture. Therefore, this compound is not urea and remains unidentified. During acclimation the changes in total free amino acid concentration in the blood of experimental mussels (Fig. 4) followed a pattern similar to the changes in blood ammonia, except that the elevation of amino acid concentration preceded the rise in blood ammonia (Fig. 3). After both 4 and 12 hours exposure to test. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 187
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