. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. COOCH3 C2iH905Br4Na; Mol. Wt. {An acid dye; absorption maxima about 520y []) There is no evidence that this dye has been furnished to biol- ogists as a stain. The alcohol soluble eosin known in the biological laboratory is apparently the following. Rio ETHYL EOSIN C. I. NO. 770 Synonyms: Eosin, alcohol soluble. Eosin S. This is similar to methyl eosin, but is the ethyl ester: Br Br NaO O. COOCHoCH, C22Hii05Br4Na; Mo
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. COOCH3 C2iH905Br4Na; Mol. Wt. {An acid dye; absorption maxima about 520y []) There is no evidence that this dye has been furnished to biol- ogists as a stain. The alcohol soluble eosin known in the biological laboratory is apparently the following. Rio ETHYL EOSIN C. I. NO. 770 Synonyms: Eosin, alcohol soluble. Eosin S. This is similar to methyl eosin, but is the ethyl ester: Br Br NaO O. COOCHoCH, C22Hii05Br4Na; Mol. Wt. {An acid dye; absorption maximum 524--527) Solubility at 26°C: in water ; in alcohol A spectral curve of this dye is given in graph 3, Fig. 24. Ethyl eosin is apparently the alcohol soluble eosin ordinarily sold by biological supply houses. For some time after the first World War it was difficult to secure this dye in America, and one stain company for a few years furnished the color acid of eosin Y when alcohol soluble eosin was ordered. (The color acids of all these dyes are alcohol soluble but almost insoluble in either hot or cold water; methyl and ethyl eosin, on the other hand, are slightly soluble in cold water but strongly soluble in hot water and alco- hol.) This error has now been corrected, and true ethyl eosin as obtainable from .American manufacturer§, is on the Commission. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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