. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYM32 LOCALIZATION IN ANTHOPLEVRA 345. Figure 3. Transmission electron micrographs of immunogold-labeled sections ol tentacles trom symbiotic Aiithnpleiim elef>uiiti.\siitui. I A) Illustrates the presence of dinoflagellates within host gastrodermal cells. (Bi Enlargement of boxed area in A. showing gold spheres associated with the multiple layers of membrane that surround the dinoflagellates. The arrows delineate the margins of the multiple membranes surrounding the dinofiagellate. Preimmune controls showed virtually no g
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYM32 LOCALIZATION IN ANTHOPLEVRA 345. Figure 3. Transmission electron micrographs of immunogold-labeled sections ol tentacles trom symbiotic Aiithnpleiim elef>uiiti.\siitui. I A) Illustrates the presence of dinoflagellates within host gastrodermal cells. (Bi Enlargement of boxed area in A. showing gold spheres associated with the multiple layers of membrane that surround the dinoflagellates. The arrows delineate the margins of the multiple membranes surrounding the dinofiagellate. Preimmune controls showed virtually no gold-sphere labeling (not shown), c == symbiont chloroplast. cw = s\rnbiont cell wall, g = gastrodermal cell of the host, s = dinoflagellate symbiont. Scale bar = 2 .m. Western Nuts The presence of gold-sphere label within the accumu- lation bodies of the symbionts suggested that the symbi- onts were producing a sym32 homolog. We performed Western analysis using anti-sym32 antiserum to look for cross-reactive proteins in homogenates from symbionts freshly removed from a host anemone and from cultured symbionts that were not in contact with host cells. Anti- sym32 Western blots of one-dimensional gels from ho- mogenates of freshly isolated Symbiodinium nniscatinei revealed three bands (Fig. 5A. lane 2). The 32-kDa band, identical in size to a band in host-only homogenate (lane 1). is probably due to contamination from host sym32. This was expected, as protein preparations of the symbi- onts are invariably contaminated by host proteins (Weis et ill., 1998). A 48-kDa band and a faint 45-kDa band below it suggest the presence of cross-reactive proteins that are produced by the symbionts. Homogenates of cultured algae (Symbiodinium bermudense) that were never in contact with host tissues (lane 3) also contained the same 45/48-kDa doublet but lacked the 32-kDa host band. Western blots of two-dimensional gels of freshly isolated 5. muscatinei homogenates revealed two spots with distinc
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
