. Auxins and plant growth. Auxin; Plant growth promoting substances. Extraction and Measurement Techniques 27 the curvature from any one concentration of auxin greatly reduced, but the shape of the concentration curve is very greatly altered (Lar- sen, 1939). Serial dilutions of auxin solutions containing inhibitors characteristically show a reduced slope, a rounded curve instead of linearity, and a lower maximum curvature. A graphic comparison of a pure auxin dilution series with an auxin-plus-inhibitor dilution series is shown in figure 12. If the presence of an inhibitor is suspected. 0
. Auxins and plant growth. Auxin; Plant growth promoting substances. Extraction and Measurement Techniques 27 the curvature from any one concentration of auxin greatly reduced, but the shape of the concentration curve is very greatly altered (Lar- sen, 1939). Serial dilutions of auxin solutions containing inhibitors characteristically show a reduced slope, a rounded curve instead of linearity, and a lower maximum curvature. A graphic comparison of a pure auxin dilution series with an auxin-plus-inhibitor dilution series is shown in figure 12. If the presence of an inhibitor is suspected. CONC. OF AUXIN Fig. 12. Dilution curves of indoleacetic acid alone and in the presence of an in- hibitor—parascorbic acid (Larsen, 1947). in a plant extract or diffusate, the establishment of a dilution curve may be extremely helpful in verifying whether an inhibitor is present or not. .Another technique for establishing the presence of an inhibitor is to test a constant concentration of indoleacetic acid with a dilution series of the extract containing the suspected inhibitor (van Overbeek et al, 1945). The presence of moderate amounts of most salts, metals, sugars, or amino acids in the agar block will have no perceptible effect on the Avena test. However, it should be pointed out that the presence of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Leopold, A. Carl (Aldo Carl), 1919-. Berkeley, University of California Press
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