. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIM domain MUSSEL LIM-1 RELATED PROTEINS Xenopus laevis Lim-1 LIM domain HOMEOBOX 265 C-terminal region Immunogen 25 I • 33 403. G T P L GB1B3H PQPGHHP S G T P L GgMD HPIPGHHPS GT P LG AIW3H PQPGHHPQ LIM1-XENLA LIM1 -MOUSE LIM1-HUMAN LIM1-BRARE 1M1-CHICK G E AQR F T D I AQR FJT D I DIMS HflPGI pITM^H pHc D S P S P E P ;HSMSAEVFG HSMSAEVFG H S M sEBlv F G SPPFSSLSVNGG YGNHLSHPP - EMNEJTAVW YGNHLSHPP~ VGNHLSHPP Y0N H L S H P P YGNHLSHPP H QJ3 L L S N U A FJ3o TGLGI I GQGGQ - G V AflT M R V l~GH!KTp S S D L sT ILIM3-XENLA G I F
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIM domain MUSSEL LIM-1 RELATED PROTEINS Xenopus laevis Lim-1 LIM domain HOMEOBOX 265 C-terminal region Immunogen 25 I • 33 403. G T P L GB1B3H PQPGHHP S G T P L GgMD HPIPGHHPS GT P LG AIW3H PQPGHHPQ LIM1-XENLA LIM1 -MOUSE LIM1-HUMAN LIM1-BRARE 1M1-CHICK G E AQR F T D I AQR FJT D I DIMS HflPGI pITM^H pHc D S P S P E P ;HSMSAEVFG HSMSAEVFG H S M sEBlv F G SPPFSSLSVNGG YGNHLSHPP - EMNEJTAVW YGNHLSHPP~ VGNHLSHPP Y0N H L S H P P YGNHLSHPP H QJ3 L L S N U A FJ3o TGLGI I GQGGQ - G V AflT M R V l~GH!KTp S S D L sT ILIM3-XENLA G I Figure 1. Multiple alignment of the deduced amino C-terminal sequence of Xlini-l with those identified from BLAST search comparisons. (A) Schematic structure of the frog Lim-1 deduced from the previously published Xliin-l sequence (Taira 1992). The C-terminal region, which was used to generate anti-XLim-1 antibodies (Karavanov el ai, 1996), is shown in black. (B) The complete sequence of the Xennpiix (XENLA) C-terminus is shown aligned with those of mouse, human, /.ebrafish (BRARE), and chick Lim-ls as well as with Xenopus Lim-5 and Lim-3 and zebrafish Lim-5. All the protein sequences were obtained from the SWISSPROT database (Bairoch and Apweiler, 2000). Black—identical amino acid residues. Dash—gaps. Comparison shows that both the size and the sequence of the XLim-1 C-terminus are highly similar (80%-90% of similarity; extent internal homology above seven amino acid residues) to that of Lim-1 proteins from other species. At the same time, the XLim-1 C-terminal sequence reveals no more than 50% of similarity (extent internal homology below seven amino acid residues) with that of Lim-5 proteins. No significant similarity was observed in the case of the .\V»i'/' Lim-3. expected by chance (Fig. 1. see Xenopus Lim-3 as an example). It is generally accepted that short peptides (below about seven amino acid residues) are of insufficient size to func- tion as immunog
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
