The Journal of biological chemistry . Casein Sut)ap STaTCh Salt Mixture 185 Bulterfat Diet?Period 118 Fig. 3. Pfeiffers bacillus added in the amount of 5 per cent to the rationseems to supply enough of the growth-stimulating factor either to maintainthe experimental animals at a constant weight or to cause them to gainweight rapidly. 900 Growth-Promoting Substances bacillus, however, three of the four test animals exhibited a distinctgain in weight, as indicated in Fig. 3. Why this organism, sosimilar to Friedlanders bacillus, should give such a differentresult we do not attempt to
The Journal of biological chemistry . Casein Sut)ap STaTCh Salt Mixture 185 Bulterfat Diet?Period 118 Fig. 3. Pfeiffers bacillus added in the amount of 5 per cent to the rationseems to supply enough of the growth-stimulating factor either to maintainthe experimental animals at a constant weight or to cause them to gainweight rapidly. 900 Growth-Promoting Substances bacillus, however, three of the four test animals exhibited a distinctgain in weight, as indicated in Fig. 3. Why this organism, sosimilar to Friedlanders bacillus, should give such a differentresult we do not attempt to explain at this time. Experiment 4-—Bacillus timothy S13,^ an acid-fast organism, wasselected for testing as the representative of this group of was found to produce an abundant pellicle, when cultivated at37°C., on veal infusion 4 per cent glycerol broth, and this medium qvamsIZSi 115105 9S Uiet Period 1 Casein Suqar Starch Salt Mixture 185 Bolter B TlTTiothu ?period 218n47310. 7dau s Fig. 4. 5 per cent of Bacillus timothy 213 was added at the point markedby the cross. It will be seen that there is a notable resumption of growthin this case. was used exclusively in this experiment. The formation of thepellicle in this case was relatively slow, and it was never possibleto filter it off in less than 10 to 12 days. The treatment of theorganisms subsequent to filtration was the same in this case asin all the other tests. Two feeding experiments were carried out,however, and the results are shown in Figs. 4 and 5. In one case5 per cent of the organisms was added to the ration and in theother 10 per cent was used to replace a like amount of starch. 2 The culture of Bacillus timothy here used is identical with Bacteriumphlei or the timothy grass bacillus of Moeller and is carried in this labora-tory as Bacillus timothy 213. Samuel R. Damon 901 Examination of the growth curves seems to indicate the presencein these organisms of a growth-promoting substance that is c
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiochem, bookyear1905
