. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HOLOTHURIAN OOCYTE MATURATION 259. FIGURE 5. Effects on the follicle cell-free oocytes of incubation mixtures of ovaries and radial nerve homogenate in H. leucospilota. Ovaries (2-3 g) were incubated with 1 ml of RNE (100 mg/ml) for 2 or 3 hours. The supernatant of the mixture was applied to follicle cell-free oocytes. Water temperature was 28°C. Bar: 100 nm. A: 0 min. B: 20 min after incubation with the supernatant. GVBD just occurs at the micropyle process. C: 110 min. Two polar bodies formed. D: follicle cell-free oocytes
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HOLOTHURIAN OOCYTE MATURATION 259. FIGURE 5. Effects on the follicle cell-free oocytes of incubation mixtures of ovaries and radial nerve homogenate in H. leucospilota. Ovaries (2-3 g) were incubated with 1 ml of RNE (100 mg/ml) for 2 or 3 hours. The supernatant of the mixture was applied to follicle cell-free oocytes. Water temperature was 28°C. Bar: 100 nm. A: 0 min. B: 20 min after incubation with the supernatant. GVBD just occurs at the micropyle process. C: 110 min. Two polar bodies formed. D: follicle cell-free oocytes incubated with RNH (50 mg/ml) for 60 min. These results show that a secondary factor which induces maturation in the follicle cell-free oocyte is produced by ovaries or testes incubated with the radial nerve factor. TABLE IV Production of a secondary factor by ovaries or testes incubated with radial nerve homogenate (RNH) or extract (RNE) No. of experiments %GVBD of oocytes in serially diluted media V, '/2 '/4 '/8 Vl6 H. leucospilota Ovaries + RNH (50 mg/ml)1 Ovaries + RNH (100 mg/ml) Ovaries + sea water RNH (100 mg/ml) Testes + RNH (100 mg/ml) Testes + sea water RNH (100 mg/ml) H. pervicax Ovaries + RNE (50 mg/ml) 4 3 3 3 2 2 2 88 ± 132 24 ± 41 98 ± 2 55 ± 34 0 ± 0 0 ± 0 0 ± 0 50 ± 21 2 ± 2 95 ± 0 0 ± 0 8 ± 2 96 ± 3 0 ±0 3 ± 2 63 ±37 12 ± 0 ± 0 - - 21 ± 30 0 ± 0 0 + 0 0 ± 0 0 ± 0 0 + 0 0 ± 0 0 ± 0 0 + 0 3 ± 3 - - 0 ± 0 - — 3 ± 2 — — 2± 3 0±0 1 The supernatant of the incubation mixture was serially diluted with sea water and assayed with the follicle cell-free oocyte from the same species. 2 Mean ± SD of GVBD oocytes observed 1 hour Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woo
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