A reference handbook of the medical sciences, embracing the entire range of scientific and practical medicine and allied science . est-tubes for thesolutions to be examined. 213 Blood Stains REFERENCE HANDBOOK, OF THE MEDICAL SCIENCES which gives a much greater absorption intensit).If it is transformed into hemochromogen, which iseasily done by dissohing a particle of the suspectedpigment in pyridin and reducing the solution soobtained with a drop of yeUow ammonium sulphide,or what is preferable, with the same amount of asaturated solution of hydrazin sulphate, about onepart of blood in 10,000
A reference handbook of the medical sciences, embracing the entire range of scientific and practical medicine and allied science . est-tubes for thesolutions to be examined. 213 Blood Stains REFERENCE HANDBOOK, OF THE MEDICAL SCIENCES which gives a much greater absorption intensit).If it is transformed into hemochromogen, which iseasily done by dissohing a particle of the suspectedpigment in pyridin and reducing the solution soobtained with a drop of yeUow ammonium sulphide,or what is preferable, with the same amount of asaturated solution of hydrazin sulphate, about onepart of blood in 10,000 parts of water can be detectedif a layer of solution one centimeter thick is examined.*For the examination of these spectra with the micro-spectroscope the fluid is conveniently placed in a C D E b i^ UO 640 »U btO UO MO flO i80 S^O ItD IfO J*0 Oo S20 . I -I -r- I . I , 1 . I . L, I, .-L ,-1 Oxjhemoglobin. Hemoglobin. AlkalineMethemoglobin. Acid Hematin. .\lkalineHematin. Hemochromogen. Neutral Hematin. Acid H ematoporphjTin. Alkaline H ematoporphyrin. Fio .817.—Absorption Spectra of Importance in theIdentification of Blood. narrow cylinder made by cementing a piece of thickwall glass tubing, one or two centimeters in length,perpendicularly on an ordinary thick microscopic slid^with Canada balsam, or temporarily with vaseline. Ifthe lumen of the tube is narrow only a very smallquantity of fluid is required to give the necessarydepth for the absorption bands. If the blood is on cloth which has been boiled,washed with soap and water, or exposed to sunlightfor a long time, the pigment may be altered into hema-tin which is insoluble in water and is best extractedby the use of ethyl ether to which one-third volumeof glacial acetic acid has been added. Hematin issoluble in this fluid as acid hematin, the spectrum ofwhich can often be recognized. If the ether is allowedto evaporate and a drop of the pyridin a
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Keywords: ., bookauthorbuckalbe, bookcentury1900, bookdecade1910, bookyear1913
