. Biological stains, a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy). are important fat staining dyes. ORANGE G. C. I. NO. 27 Synonym: Wool orange 2G. Slightly different grade: Orange GG, GMP. S03Na /\_N = N I I ; HO" SOjXa (An acid dye; absorption maximum about ) This dye is strongly acid because of the two sulphonic groups. It is one of the most valuable plasma stains in histological work. It has great use as a background stain for haematoxylin and other nuclear dyes in cytology. It is frequently employed, both b
. Biological stains, a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy). are important fat staining dyes. ORANGE G. C. I. NO. 27 Synonym: Wool orange 2G. Slightly different grade: Orange GG, GMP. S03Na /\_N = N I I ; HO" SOjXa (An acid dye; absorption maximum about ) This dye is strongly acid because of the two sulphonic groups. It is one of the most valuable plasma stains in histological work. It has great use as a background stain for haematoxylin and other nuclear dyes in cytology. It is frequently employed, both by botanists and zoologists, as a cytoplasmic stain, together with the two nuclear dyes safranin and gential violet in the Flemming triple stain. It is of importance to the pathologist for its use with anilin blue and acid fuchsin in the Mallory connective tissue stain; and is used in various other double and triple staining methods, such as that of Ehrlich-Biondi-Heidenhain, in which it is mixed with methyl green and acid fuchsin. The Erhlich "triacid mixture," also a combination of these same three dyes, is used in staining blood. A further use is Bensley's "neutral gentian," a combination of orange G and gential violet for staining the islands of Langer- hans. 34. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975; Ambler, Joseph Alfred, 1889-; Kornhauser, S. I. (Sidney Isaac), 1887-1959; Mallory, Frank Burr, 1862-1941; Sharp, Lester W. (Lester Whyland), b. 1887. Geneva, N. Y. , The Commission
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