. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. NO. NaSOj SO3 Na Ci6H8N40iiS2Na2; Mol. Wt. {An acid dye) This dye has recently been proposed as an indicator, having a pH range of to Its acid color is bright yellow, from which it passes thru gray-green into its alkaline color, bright indicator can be used in the form of test strips, like litmus paper. It is more sensitive than litmus, however, and shows greater color contrast. Lillie (1940) finds it c
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. NO. NaSOj SO3 Na Ci6H8N40iiS2Na2; Mol. Wt. {An acid dye) This dye has recently been proposed as an indicator, having a pH range of to Its acid color is bright yellow, from which it passes thru gray-green into its alkaline color, bright indicator can be used in the form of test strips, like litmus paper. It is more sensitive than litmus, however, and shows greater color contrast. Lillie (1940) finds it can replace xylidine ponceau in the Masson (1929) trichrome stain. For technic of Masson trichrome stain, Lillie modification, see Staining Pro- cedures, p. IB3-I8. cSl SUDAN II C. I. NO. 73 Synonyms: Oil scarlet. Fast oil orange II. Red B. Fat ponceau. Orange C18H16N2O; Mol. Wt. {An acid dye; absorption maxima in alcohol: ) Solubility at 26°C: in water nil; in alcohol In this dye the hydroxyl group is in the ortho position with re- spect to the azo group. As explained above (p. 69), such com- pounds show a tendency toward intramolecular rearrangement so that the hydrogen atom detaches itself from the hydroxyl group and becomes fixed to the neighboring nitrogen. Such a com- pound is neither acid nor basic and, not being able to form salts, is not an ordinary dye, but is fat soluble and has the power of color- ing fat. Therefore, this compound, together with the following and several others of similar structure described below, are fat stains. This fat stain has frequently been confused with oil red O be- cause both are mistakenly given in the Colour Index as Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech
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