The Journal of biological chemistry . 15 5 6 7 8 808 Electrolj^te and Water Distribution was completed, and making a correction for the slight amountof CO2 formed in that time. However, the proteins themselvesappear to be affected by oxidation during the saturation, andperhaps during the dialysis, and to lose some of their alkali-bin
The Journal of biological chemistry . 15 5 6 7 8 808 Electrolj^te and Water Distribution was completed, and making a correction for the slight amountof CO2 formed in that time. However, the proteins themselvesappear to be affected by oxidation during the saturation, andperhaps during the dialysis, and to lose some of their alkali-binding power. Some loss of oxygen-binding power (methemo-globin formation?) also occurs during dialysis. We consequentlyperformed both diatysis and CO2 capacity determinations onreduced solutions, and made the dialysis as brief as possible. Thecell solution was saturated with pure CO2 and dialyzed in nar-row collodion tubes at 20°C. for 2 days against a great volume(100 liters) of distilled water, which was agitated by a stirrer. Fig. 8. Equivalents of base bound per gram of serum protein. The water was changed every 12 hours. We attempted in thismanner to attain the necessary purification with as short a dialy-sis as possible. In the 2 day period we did not succeed in lowering the con-ductivity so far as in the longer diahses used with the serumproteins. A choice had to be made, however, of a dialysis periodsuch that the error due to alteration of hemoglobin should not begreater than that due to retained alkali. The saturators were filled with mixtures of hydrogen and CO2instead of air and CO2. In order to make reductions as completeas possible the solution (10 cc.) was introduced into the saturator, Van Slyke, Wu, and McLean 809 and the latter was evacuated, filled with hydrogen, and evacuatedagain. It was then rotated, so that the blood was distributedabout the walls and the oxygen could be removed. The saturatorwas refilled with hydrogen and evacuated with a water pump
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