. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 144 K. NAKAYAMA ET AL. 17kd protein; 1 10 20 I-G-H-S-G-N-G-L-V-Y-Y-G-N-H-A-Y-L-F-S-A 21 25 -D-H-L-T-G 1 10 20 protein; Y-D-F-S-F-X-K-K-R-N-D-G-F-Y-A-D-P-A-S-H 21 30 -Q-N-Y-Y-R-R-V-S-G-A Figure 2. Amino acid sequences of the 17-kDa and proteins in the morula-like cell. The one- letter code is used. X. unidentified. detection techniques. Immunoreactive protein appeared as a broad band and a slightly smaller component (ca. 5 kDa) that migrated faster than kDa (Fig. 5A, lane 2). The 16-kDa and proteins
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 144 K. NAKAYAMA ET AL. 17kd protein; 1 10 20 I-G-H-S-G-N-G-L-V-Y-Y-G-N-H-A-Y-L-F-S-A 21 25 -D-H-L-T-G 1 10 20 protein; Y-D-F-S-F-X-K-K-R-N-D-G-F-Y-A-D-P-A-S-H 21 30 -Q-N-Y-Y-R-R-V-S-G-A Figure 2. Amino acid sequences of the 17-kDa and proteins in the morula-like cell. The one- letter code is used. X. unidentified. detection techniques. Immunoreactive protein appeared as a broad band and a slightly smaller component (ca. 5 kDa) that migrated faster than kDa (Fig. 5A, lane 2). The 16-kDa and proteins in the morula-like cells (Fig. 5B, lane 1) and the immunoreactive protein in the zooxanthellae (Fig. 5B, lane 2) were shown to be glycosylated. Discussion Molluscan hemocytes packed with large colorless gran- ules have been observed only in giant clams (the Tridacni- 12345. -MGkDa -* Figure 3. Immunoblot analysis of the protein specific to the morula-like cell. Hemocytes and the Percoll density-gradient frac- tions were electrophoresed, electrotransferred onto PVDF membrane, and immunologically stained. Lane 1. hemocytes lysate; Lane 2. lysate of eosinophilic granular hemocytes and agranular cells; Lane 3, morula- like cell lysate; Lane 4. precipitate of Percoll density gradient centrifuga- tion of the morula-like cell lysate; Lane 5, the large granule lysate. Each lane contained ^g protein except lane 5, which contained //g protein. dae). This type of hemocyte is known as a type II cell in Tridacna maxima (Reade and Reade, 1976) and a morula- like cell in T. crocea (Nakayama et ai, 1997). Proteins with molecular weights of 64 kDa, 17 kDa and kDa were specific to the morula-like cell of T. derasa and not associated with the eosinophilic granular hemocyte or the agranular cell. The protein is contained in the large granules. No homologous sequence was found in the homology search of the N-terminal amino acid se- quences of these proteins (Fig.
Size: 1650px × 1513px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
