. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Q> O 0 1 ih. 1 2 3 4 5 6 7 8 9 10 11 12 Fraction number Figure 3. Percoll density-gradient centrifugation of intestinal epithelial cells of Aiigni/lu luponicu. Pro- cedures for separation are described in Materials and Methods. (•). Number of total cells; (D), number of cells that incorporated FITC-conjugated dextran; (D). number of 8Fl-positive cells; (—), density of Percoll. formes. Vigneulle and Laurencin (1991) showed that for- malin-fixed Vibrio anguillariini was ingested by entero-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Q> O 0 1 ih. 1 2 3 4 5 6 7 8 9 10 11 12 Fraction number Figure 3. Percoll density-gradient centrifugation of intestinal epithelial cells of Aiigni/lu luponicu. Pro- cedures for separation are described in Materials and Methods. (•). Number of total cells; (D), number of cells that incorporated FITC-conjugated dextran; (D). number of 8Fl-positive cells; (—), density of Percoll. formes. Vigneulle and Laurencin (1991) showed that for- malin-fixed Vibrio anguillariini was ingested by entero- cytes of rainbow trout Oncorhynchys rnykiss, sea bass Di-. Figure 4. Double-label, immunofluorescence micrograph of an in- testinal epithelial cell of Anguilla japonica incorporating rabbit IgG in vivo. Rabbit IgG ( mg) was injected into the lumen of the intestine. Thirty minutes later, intestinal cells were separated, fixed, and treated with methanol. After being labeled with 8F1, the cells were double-labeled with rhodamine-conjugated goat anti-mouse Ig(G + M) and FITC-con- jugated goat anti-rabbit IgG. (A) A cell containing rabbit IgG visualized with the FITC-conjugated antibody; (B) the same cell harboring the 8F1 - antigen proteins, identified by the labeling with the rhodamine-conjugated antibody. Bar, 5 urn. centrarchus lahrax, and turbot Scophthalmus muxinnts after oral administration or anal intubation. To our knowledge, the present paper is the first to show that active endocytic cells occur in the intestine of the adult eel. The aim of this study was to prepare monoclonal antibodies to probe for cells undergoing active endocytosis in the eel intestine. We succeeded in obtaining a monoclonal an- tibody, 8F1, which specifically recognized one type of en- docytic cell. We made a survey of cells harboring 8F1- reactive proteins in the histological sections that had been prepared from the intestine of various species belonging to Cluperiformes, Myctophiformes, Cypr
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