. Bacteriology and mycology of foods. Food. 18 BACTERIOLOGICAL APPARATUS placed on its support, introduce the NaOH by means of a pipette into the bottom of the tube and seal the tube immediately with a rubber stopper. Wright's Method, This is another absorption method and is different from the others only in method of procedure. It is especially adaptable to the isolation of anaerobic spore-forming bacteria. Decimal dilutions of the sample are introduced into sterile litmus milk. About 1 in. above the meniscus of the milk a tight cotton cylinder is forced upon which are put about 2 gms. of pyr
. Bacteriology and mycology of foods. Food. 18 BACTERIOLOGICAL APPARATUS placed on its support, introduce the NaOH by means of a pipette into the bottom of the tube and seal the tube immediately with a rubber stopper. Wright's Method, This is another absorption method and is different from the others only in method of procedure. It is especially adaptable to the isolation of anaerobic spore-forming bacteria. Decimal dilutions of the sample are introduced into sterile litmus milk. About 1 in. above the meniscus of the milk a tight cotton cylinder is forced upon which are put about 2 gms. of pyrogallol. This is treated with strong NaOH and the original plug replaced in the tube. This tube should then be heated for fifteen minutes at 80° to kill all vegetative bacteria. Later incubation will give the characteristic changes. Lentz's Method. This requires a glass plate 125 mm. square and cellulose absorbent rings. Before using the rings are soaked in pyrogal- lic acid and immediately before the culture is started in 1 per cent potassium hydroxide. The plate with the culture is inverted over the cellulose ring, being sealed to the glass with Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Tanner, Fred Wilbur, 1888-1957. New York, John Wiley & Sons, Inc. ; [etc. ,etc. ]
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