. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYPOPHYSECTOMIZED FUNDULUS 11 in a standing tank from addition of food, evaporation, etc. As the only fresh water readily available was from the municipal water supply, three problems presented themselves in designing the required system: constant rate of flow, constant tem- perature, and dechlorination. The arrangement is shown in Figure 1. Tap water was received into a constant level tank (A) placed above the aquaria. The water flowed from the tank by gravity at a rate regulated by a clamp (B) through a series of ten five-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYPOPHYSECTOMIZED FUNDULUS 11 in a standing tank from addition of food, evaporation, etc. As the only fresh water readily available was from the municipal water supply, three problems presented themselves in designing the required system: constant rate of flow, constant tem- perature, and dechlorination. The arrangement is shown in Figure 1. Tap water was received into a constant level tank (A) placed above the aquaria. The water flowed from the tank by gravity at a rate regulated by a clamp (B) through a series of ten five-gallon car- boys (C 1-10). The first two contained activated charcoal in sausage skins (D 1 and 2) to remove dissolved chlorine (modified method of Coventry, Shelford and FIGURE 1. Running fresh water system (drawn by Patricia J. Harris). Miller, 1935). The outflow from the last carboy was dripped into an aerated con- tainer (E) for the purpose of reoxygenation. From here water was fed into the aquaria (G 1 and 2) by means of a constant level siphon (F 1), a similar arrange- ment being used to adjust the outflow (F 2). The flow of water was regulated to deliver ten gallons a day to each of two one- gallon aquaria, thereby giving a turnover rate of about once in every two and one- half hours. The battery of carboys created a reservoir such that it took over two days for water to pass through the system. During this time it reached aquarium room temperature wrhich was regulated at ca. 15° C. Tests of the outflow with orthotolidine indicator showed it always to be chlorine-free. The only difficulty encountered was bubbles in the reservoir lines brought about by warming of the water. Flow irregularities due to these were easily avoided by removal of one of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
