. Carnegie Institution of Washington publication. . Fig. 47.* Fig. Fig. forms of tubes should not be used. One of these, a short, thick tube with a wide U, in use in some laboratories in this country, allows air to pass readily into the closed end and is entirely worthless. A sample tube of this sort was filled with *Fic. 47.—Fermentation-tube with Bacillus tracheiphilus, showing absence of gas and uniform clouding in open and closed end in the presence of grape-sugar. The fluid consisted of water, 400; Savory & Moore's peptone, 4; sodium chloride, i; c. p. grape-sugar, 2; satura


. Carnegie Institution of Washington publication. . Fig. 47.* Fig. Fig. forms of tubes should not be used. One of these, a short, thick tube with a wide U, in use in some laboratories in this country, allows air to pass readily into the closed end and is entirely worthless. A sample tube of this sort was filled with *Fic. 47.—Fermentation-tube with Bacillus tracheiphilus, showing absence of gas and uniform clouding in open and closed end in the presence of grape-sugar. The fluid consisted of water, 400; Savory & Moore's peptone, 4; sodium chloride, i; c. p. grape-sugar, 2; saturated solution carbonate of soda (20° C.), 20 drops, i. e., enough to render the fluid slightly alkaline to litmus. tFic 48.—Fermentation-tube with Bacillus tracheifhilus, showing inability of organism to grow anaerobically with glycerin as the carbon food. Fluid, distilled water wjth I per cent Witte's pep- tonum siccum and I per cent Schering's c. p. glycerin. Copious growth in open end and in outer part of U; none in the closed end. jFic. 49.—Fermentation-tube of cane-sugar peptone water inoculated with a white, gas-forming organism plated from a spot disease of sisal hemp. The total amount of gas produced and its rate of evolution at 20° to 23° C. are indicated by marks on the closed end of the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Carnegie Institution of Washington. Washington, Carnegie Institution of Washington


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