. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 130 Fig. 64.—Pasteur's Flask. Pastern's Apparatios.—Special forms of tubes, bulbs, and pipettes are employed by the school of Pasteur. The tubes are provided with lateral or with curved arms drawn out to a fine point, and with slender necks plugged with cotton-wool. A double form (Pig. 65) shaped like a tuning- fork, each limb with a bent arm, is con- venient for storing sterihsed broth. The sealed end of
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 130 Fig. 64.—Pasteur's Flask. Pastern's Apparatios.—Special forms of tubes, bulbs, and pipettes are employed by the school of Pasteur. The tubes are provided with lateral or with curved arms drawn out to a fine point, and with slender necks plugged with cotton-wool. A double form (Pig. 65) shaped like a tuning- fork, each limb with a bent arm, is con- venient for storing sterihsed broth. The sealed end of an arm is nipped off with sterilised forceps, the sterile broth aspirated into each limb, and the arm again sealed in the flame; a series of such tubes can be arranged upon a rack on the working table. Bulbs with a vertical neck drawn out to a fine point, others with a neck bent at an obtuse angle, plugged with cotton- wool, and a lateral curved arm drawn out to a fine point, are also employed. For a description of these various vessels and their special advantages, the woi-ks of PasteiTr and Duclaiix must be con- „ , ^ Fig. 65.—Pasteue's Double suited. Tpbj,. (h) Cultivation of Anaerobic Bacteria. To cultivate anaerobic organisms the same media are employed as for aerobic oi-ganisms, but the methods must be modified, or special apparatus used, so that the oxygen in the air may be excluded. In the preparation of plate-cultivations, before the film of gelatine has completely hardened it is covered with a sheet of mica, and the edges are sealed with melted paraiiine. By this process the air is not completely excluded, so that only those organisms which are not strictly anaerobic can be grown by this method. Liborius recom- mends boiling a considerable volume of gelatine in a tube, cooling it^ and after thoroughly distributing the organisms in the still liquid jelly, rapidly solidifying it by placing the tube in iced water. By this. Please note that these images are extra
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