. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LENS DIFFERENTIATION 199 pBp. Ps a PH PL V Ps FIGURE 8. SDS polyacrylamide gel electrophoresis of Sephadex G 200 separated bovine lens crystallin subunits (after Bloemendal, 1981). B5 is a low molecular weight beta (27,000 daltons) that resembles gamma components 2 and 3. membranes are illustrated in Figure 11 (Specter et a/., 1979; Broekhuyse, 1981). Not only do the cytoplasmic elements differentiate as lens epithelial cells become fiber cells, but the cell membranes also change substantially in this way. It is clear that w
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LENS DIFFERENTIATION 199 pBp. Ps a PH PL V Ps FIGURE 8. SDS polyacrylamide gel electrophoresis of Sephadex G 200 separated bovine lens crystallin subunits (after Bloemendal, 1981). B5 is a low molecular weight beta (27,000 daltons) that resembles gamma components 2 and 3. membranes are illustrated in Figure 11 (Specter et a/., 1979; Broekhuyse, 1981). Not only do the cytoplasmic elements differentiate as lens epithelial cells become fiber cells, but the cell membranes also change substantially in this way. It is clear that with the loss of cytoplasm and organelles as fiber cells are relocated toward the lens core, the fiber cell membranes collect much bound non-membrane proteins. This increases the protein-to-lipid ratio so as to produce an abnormal membrane environment. There is also a dehydration in the nuclear core as compared with the outer portions of the lens. Another important influence on fiber cell membranes relative to differentiation is the accumulation of formerly water-soluble proteins in an aggregated form and an unfolding of these proteins so as to expose reactive side groups and to stimulate unnatural protein:protein interactions. The exposure of SH groups is an example of this process, and it has been shown by many investigators (Roy and Spector, 1976; Bloemendal, 1981). Spector et al. (1979) have shown that a protein of 23,000 dalton molecular weight has a special affinity for membrane surface proteins in human. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marin
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