. A manual of bacteriology. Bacteriology. io8 MANUAL OF BACTERIOLOGY. tion is concerned, in case samples of urine are to be sent by mail; thymol should not be added if cultures are to be made. Specimens of sputum, pus or blood may be collected con- veniently in the form of thin smears upon cover-glasses. The smears are fixed by passing through the flame three times. Smears of blood are prepared as follows: Have two perfectly clean, square cover-glasses. The finger, or the lobe of the ear, having been carefully washed with water, alcohol and ether, is punctured with a sterilized needle, and a s
. A manual of bacteriology. Bacteriology. io8 MANUAL OF BACTERIOLOGY. tion is concerned, in case samples of urine are to be sent by mail; thymol should not be added if cultures are to be made. Specimens of sputum, pus or blood may be collected con- veniently in the form of thin smears upon cover-glasses. The smears are fixed by passing through the flame three times. Smears of blood are prepared as follows: Have two perfectly clean, square cover-glasses. The finger, or the lobe of the ear, having been carefully washed with water, alcohol and ether, is punctured with a sterilized needle, and a small drop of blood issues which is wiped away with a clean cloth. The second drop of blood should be taken; it should be about the size of a pin's head. No pressure should be exerted upon the skin. This drop of blood is placed on one of the cover-glasses. The other cover-glass is laid upon the first, both being handled with for- ceps. The drop of blood becomes flattened out into a thin film. Immediately and be- fore the blood has had time to coagulate the two are slipped or slid away from each other in a horizontal plane, not forcibly pulled apart. In this way the blood will be spread in thin films on the cover-glasses. It is best to place the cover-glasses so that one does not cover the other exactly, but so that the sides of the one lie diagonally to the sides of the other, although their centers coincide (Fig. 37). Films of blood which are to be examined for the parasite of malaria may be prepared in this manner. Drops of blood to be used for the serum reaction for typhoid fever must be large-sized. They may be collected on cover-glasses or pieces of unsized paper and allowed to dry. In place of drops of blood caught in this manner, the use of capillary tubes for collecting the blood are to be preferred, since by this method it is possible to obtain the serum free from corpuscles after the clot forms in the tube. To test blood by culture methods. Fig. 39.—Manner of placing
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