. Biological structure and function; proceedings. Biochemistry; Cytology. 64 GERTRUDE E. PERLMANN TABLE III Rotatory Dispersion Constant, A,., and Specific Activity of Pepsin in Various Solvents as Function of Temperature Relative specific Composition of solvent pH* Temperature A^ activity per unit nitrogen in per centf o • I M Acetate buffer 4-34 23 2l6 100 55 227 99 8 • o M Urea-acetate 5-35 23 2X8 95 55 229 5 5 -o M Guanidine hydrochloride 3-So 23 217 100 55 226 4 * Apparent pH. t The relative specific activity of a freshly prepared pepsin solution in o • i M acetate buffer of pH 46 is take
. Biological structure and function; proceedings. Biochemistry; Cytology. 64 GERTRUDE E. PERLMANN TABLE III Rotatory Dispersion Constant, A,., and Specific Activity of Pepsin in Various Solvents as Function of Temperature Relative specific Composition of solvent pH* Temperature A^ activity per unit nitrogen in per centf o • I M Acetate buffer 4-34 23 2l6 100 55 227 99 8 • o M Urea-acetate 5-35 23 2X8 95 55 229 5 5 -o M Guanidine hydrochloride 3-So 23 217 100 55 226 4 * Apparent pH. t The relative specific activity of a freshly prepared pepsin solution in o • i M acetate buffer of pH 46 is taken as 100. 230 5*1: 225 ^^ o o u O 220 • 0-In acetate buffer - D 8-Om urea A 5'Om guanidine hydrochloride \i. -g---^n 100 80 60 40 20 20 30 40 50 Temperature ( C) 60 Fig. 4. Dependence of temperature of the optical rotatory dispersion constant, A,,, and enzymic activity of pepsin in various solvents. segment necessary for the biological activity of the enzyme has been affected. From the results presented here, one can conclude: I. Although hydrogen bonds undoubtedly exist in pepsin, they are relatively unimportant in maintaining the configuration of the protein essential for its enzvmic Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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